Agents neoplastic transformation

Lubet RA, Kiss E, Gallagher MM. et al. 1983b. Induction of neoplastic transformation and DNA single-strand breaks in C3H/10T1/2 clone 8 cells by polycyclic hydrocarbons and alkylating agents. J Natl Cancer Inst 71 991 -998. 

A recent report has shown that DNA hypomethylation may contribute to the induction of tumors in mice via an epigenetic mechanism causing chromosomal instability. In addition to facilitating neoplastic transformation, alterations in DNA methylation patterns have been shown to exert effects on embryonic development and acquired drag resistance to cytotoxic agents. Recent data from oiu laboratory have demonstrated the... 

Apoptosis is a major mechanism by which many current synthetic anticancer and chemotherapeutic agents induce their effect. As such, apoptosis has emerged as an important mechanism by which dietary compounds may exhibit chemopreventive potential. Apoptosis may remove cells undergoing neoplastic transformation when other cellular defense systems have failed to block the carcinogenesis process upstream. Removing the neoplastic cells by induction of apoptosis represents a cornerstone of the chemopreventive paradigm [134]. 

Camptothecin (Fig. 4), a quinoline alkaloid, is a potent anti-neoplastic agent that inhibits DNA topoisomerase I. Accumulation of up to 8 mg of camptothecin/L was reported for hairy root cultures of Ophiorriza pumila transformed with A. rhizogenes. Cotyledon-derived calluses of Nothapodytes foetida were able to produce not only camptothecin, but also its derivatives also known to exhibit anticancer activity. 

Syrian hamster embryo (SHE) cell transformation assay (In vitro) (SHE cells are used to evaluate the potential of a wide range of agents to induce morphological transformations. Following carcinogen exposure (1-7 days), SHE cells display a multistage pattern of neoplastic progression similar to that observed in vivo)... 

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