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Hapten-immobilizing affinity column

Fig. 4. Classification of reported noncompetitive immunoassays for haptens based on the assay principle. (A) Assays that include a chemical modification of hapten to allow sandwich-type detection. (B1) Improved single-antibody immunometric assays that separate immune complex and excess labeled antibody, either by using a hapten-immobilized affinity column or based on differences in their physical properties. (B2) A variation of single-antibody immunometric assays based on masking of unoccupied antibody by an immunoreactive macromolecule followed by selective capture and detection of the hapten-occupied antibody. (C) Assays employing a probe molecule specific to a hapten-antibody complex. Fig. 4. Classification of reported noncompetitive immunoassays for haptens based on the assay principle. (A) Assays that include a chemical modification of hapten to allow sandwich-type detection. (B1) Improved single-antibody immunometric assays that separate immune complex and excess labeled antibody, either by using a hapten-immobilized affinity column or based on differences in their physical properties. (B2) A variation of single-antibody immunometric assays based on masking of unoccupied antibody by an immunoreactive macromolecule followed by selective capture and detection of the hapten-occupied antibody. (C) Assays employing a probe molecule specific to a hapten-antibody complex.
Such a hapten-immobilizing affinity column was also operated on-line as part of a high-performance liquid chromatography (HPLC) system (13). This is a... [Pg.153]

See other pages where Hapten-immobilizing affinity column is mentioned: [Pg.139]    [Pg.151]    [Pg.139]    [Pg.151]    [Pg.153]    [Pg.153]    [Pg.153]    [Pg.154]    [Pg.101]    [Pg.101]    [Pg.802]    [Pg.26]    [Pg.355]    [Pg.109]    [Pg.334]    [Pg.520]    [Pg.334]   
See also in sourсe #XX -- [ Pg.151 , Pg.152 , Pg.153 ]



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