Affinity-based mass spectrometry

M. A. Kelly, T. J. McLellan, and P. J. Rosner, Strategic use of affinity-based mass spectrometry techniques in the drug discovery process, Anal. Chem. 74 (2002), 1-9. 

Kelly, M.A. Mclellan, T.J. Rosner, PJ. Strategic Use of Affinity-Based Mass Spectrometry Techniques in the Drug Discovery Process, Anal. Chem. 74, 1-9 (2002). 

Strategic use of affinity-based mass spectrometry techniques in the drug discovery process. Anal Chem 74 1-9. 

Chen, W.-Y, Chen, Y.-C. (2006) Affinity Based Mass Spectrometry by Using Iron Oxide Magnetic Particles as the Matrix and Concentrating Probes for SALDI MS Analysis of Peptides and Proteins. Anal. Bioanal. Chem. 386 699-704. 

ALIS An Affinity Selection-Mass Spectrometry System based on Continuous SEC... 

Several affinity screening methodologies that include MS-based readout and work under protein-excess conditions have been developed in the past decade [1]. Some examples include affinity selection/mass spectrometry (ASMS Abbott Labs [10]), size exclusion chromatography with LC-ESI-MS (see Chapter 2 and 3 [11-19]), the use of coupled or non-coupled pulsed ultra-filtration/mass spectrometry (summarized in this chapter [11, 20-23]), restricted access phase chromatography (see Chapter 5 [24, 25]), capillary electrophoresis [26, 27], target shift mass spectrometry [28], and multitarget affinity/specificity screening (MASS, see Chapter 10 [29, 30]). 

Annis, A. Genome-Scale Drug Discovery by Affinity Selection-Mass Spectrometry-Based Screening of Mass-Encoded Small Molecule Libraries, ... 

Affinity mass spectrometry 9 Atmospheric pressure mass spectrometry 4 Desorption/ionization mechanism 2 Enzymatic activities measurement 6 Mass Spectrometry Imaging (MSI) 5 Mass-spectrometry detection 3 Nanostructure-initiator mass spectrometry (NIMS) 5 Porous silicon based mass spectrometry 9... 

Guerrero C, Tagwerker C, Kaiser P, et al. An integrated mass spectrometry-based proteomic approach quantitative analysis of tandem affinity-purified in vivo cross-linked protein complexes (QTAX) to decipher the 26 S proteasome-interacting network. Mol. Cell. Proteomics. 2006 5 366-378. 

Ionization may take place by the interaction with a particle sufficiently high in energy, e.g. an electron or a photon, or by the addition of charged species, e.g. an electron or a proton. The thermochemistry associated with the ionization process provides information on ion structures, since a structure may be assigned based on heat of formation when compared to data of reference ions. Thus, the determination of ionization energy, electron affinity and proton affinity plays a central role in mass spectrometry. 

Kaur, S., McGuire, L., Tang, D., Dollinger, G., Heubner, V. Affinity selection and mass spectrometry-based strategies to identify lead compounds in combinatorial libraries. J. Prot. Chem. 1997, 16, 505-511. 

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