Affinity selection-mass spectrometry

ALIS An Affinity Selection-Mass Spectrometry System for the Discovery and Characterization of Protein-Ligand Interactions... 

E. A. An affinity selection-mass spectrometry method for the identification of small molecule ligands from self-encoded combinatorial libraries, discovery of a novel antagonist of E. coli dihydrofolate reductase. Int. J. Mass. Spectrom. 2004, 238, 77-83. 

Several affinity screening methodologies that include MS-based readout and work under protein-excess conditions have been developed in the past decade [1]. Some examples include affinity selection/mass spectrometry (ASMS Abbott Labs [10]), size exclusion chromatography with LC-ESI-MS (see Chapter 2 and 3 [11-19]), the use of coupled or non-coupled pulsed ultra-filtration/mass spectrometry (summarized in this chapter [11, 20-23]), restricted access phase chromatography (see Chapter 5 [24, 25]), capillary electrophoresis [26, 27], target shift mass spectrometry [28], and multitarget affinity/specificity screening (MASS, see Chapter 10 [29, 30]). 

We have developed a high throughput ultrafiltration affinity screening method coupled to MS (affinity selection/mass spectrometry ASMS), which works with any soluble target and small molecule library (including natural products). ASMS is amenable to parallelization, efficient and robust enough to allow study... 

Annis A, Chuang C-C, Nazef N An affinity selection-mass spectrometry system for the discovery and characterization of protein-ligand interactions, chapter 3. 

Annis, D. A., Nickbarg, E., Yang, X., Ziebell, M. R., Whitehurst, . E. (2007) Affinity selection-mass spectrometry screening techniques for small molecule drug discovery. Curr Opin Chem Biol 11, 518-526. 

Annis D.A., Athanasopoulos J., Curran P.J., Felsch J.S., Kalghatgi K., Lee W.H., Nash H.M., Orminati J.P.A., Rosner K.E., Shipps GW., Thaddupathy G.R.A., Tyler A.N., Vilenchik L., Wagner C.R., Wintner E.A., An affinity selection-mass spectrometry method for the identification of small molecule ligands from self-encoded combinatorial libraries. Discovery of a novel antagonist of E. coli dihydrofolate reductase Int. J. Mass Spectrom. 2004, 238, 77-83. 

Figure 7-12. Diagram of the automated ligand identification system (ALIS) affinity selection-mass spectrometry method. (Reprinted from reference 85, with permission of Elsevier Science.)...

Yang, H. Cheng, X. Bakhoum, A. Hajduk, P Lico, L Voorbach, M. Dand-liker, P Schurdak, M. Gao, L. Buko, A. Miesbauer, L. Schmitt, R. Martin, Y Beutel, B. Burns, D. Use of Affinity Selection Mass Spectrometry to Screen Organic Compounds of Diverse Structures for Drug Leads, Abstracts of Papers, American Chemical Society (2001), 221st ANYL-192. 

Annis, A. Genome-Scale Drug Discovery by Affinity Selection-Mass Spectrometry-Based Screening of Mass-Encoded Small Molecule Libraries, ... 

Library Affinity Selection-Mass Spectrometry (LAS-MS) In this approach the library components are reacted with agarose gel-bound receptor in solution [101]. After washing the beads, peptides of increasing receptor affinity are released by adding to the beads elution buffers of different pH values the peptides released are identified with ESI-MS or ESI-MS/MS. 

Adam GC, Parish CA, Wisniewski D, Meng J, Liu M, Calati K, Stein BD, Athanasopoulos J, Liberator P, Roemer T, Harris G, Chapman KT (2008) Application of Affinity Selection/ Mass Spectrometry to Determine the Structural Isomer of Pamafungins Responsible fra-Binding Polyadenosine Polymerase. J Am Chem Soc 130 16704... 

Affinity selection - mass spectrometry screening techniques for... 

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