Adsorption chromatography serum

Thiophilic adsorption chromatography has been described for the purification of murine monoclonal antibodies from hybridoma cell culture containing fetal bovine serum.154 Due to the very low concentration of immunoglobulins in cell culture supernatants, binding capacity remains modest in spite of the presence of 0.5 to 1 M potassium sulfate. Further developments of this technology described by Nopper et al.155 indicated that thiophilic sorbents could be modified in their structure to increase the specificity and the binding capacity. 

Extraction of blood and serum follows pretreatment of the serum with methanol, and extraction is performed with a hexane/ether mixture. This method described by Burse et al. [30] is a standard extraction method for blood and serum, and has been used in most studies. Florisil columns are used in the sample-clean up. An analytical method was developed to quantitate PCBs and PBBs in human serum. This method includes a hexane-ethyl ether extraction of methanol-denatured serum, and an adsorption chromatography with deactivated silica gel [31]. 

Increasing use of Sephadex G-types, LH-20 and Bio-Gel P types for adsorption chromatography has been made. For example a number of methods have been reported for the determination of oestrogens, from pregnancy urine on G-15 [197] and from plasma on LH-20 [198]. Xanthurenic acid in urine has been determined using a procedure based on adsorption to Sephadex [199] and serum uric acid has been purified by adsorption to Bio-Gel P-2 [200]. The binding of thyroid hormones to Sephadex is well known [201] and forms the basis of a number of thyroid function tests [202, 203]. A number of kits based on Sephadex are commercially available and some of these kits were recently compared [204],... 

Porath, J., and Olin, B. (1983) Immobilized metal ion affinity adsorption and immobilized metal ion affinity chromatography of biomaterials. Serum protein affinities for gel-immobilized iron and nickel ions. Biochemistry 22, 1621-1630. 

Anti-a-D-mannose antibodies have been isolated from the serum of rabbits immunized with the glycoconjugate of a-D-mannose and bovine serum albumin [85], The antibodies were purified by affinity chromatography with adsorption on a mannosyl-Sepharose column and elution with a-D-mannose or methyl a-D-mannoside. Such antibodies should be especially useful for studying the detection of diseases due to the appearance of abnormal glycoproteins that contain mannose polymers. 

Choline bromide succinate, 998 Choline chloride, 466 Choline chloride carbamate, 427 Choline chloride succinate, 998 Choline citrate, 466 Choline dihydrogen citrate, 466 Choline salicylate, 965 Choline theophyllinate, 1011 Cholinesterase activity, assay kit, 1171 quantification in serum, 23 test for inhibition of, 6 Chondodendron tomentosum, 1057 Chromatographic performance, evaluation of, 189 Chromatography, adsorption, 204... 

Another technique that allows for the direct injection of plasma or serum online with the chromatographic system uses an analytical coliunn containing particles referred to as restricted-access media (RAM) traditional laminar flow liquid chromatography is employed. These RAM particles are designed to prevent or restrict large macromolecules from accessing the inner adsorption sites of the bonded phase. Commercially available RAM columns, all... 

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