Adriamycin resistant cell lines

T.media possesses antitumour activity (e.g. IL against L1210 murine leukemia cells of 3.6 0.3 nM) higher than that of taxol (e.g. 57.0 3.0 nM) and is also active against adriamycin resistant cells (32). Taxol C possesses selective cytotoxicity in the NCI human cell line screen (33). 10-Deacetyltaxol C, A -methyltaxol C (130) and taxol D (133) also showed activity close to that of taxol in the tubulin assembly assay (32,33). Table 1 lists the different taxanes isolated thus far from different Taxus species. 

Li J, Xu LZ, Yao JJ, Guo WJ, Xia P, Chen Y (2001a) Reversal effects of droloxifene on multidrug resistance in adriamycin-resistant K562 cell line. Acta Pharmacol Sin 22(11) 1023—1027... 

Mirski, S.E., Gerlach, J.H. and Cole, S.P. (1987) Multidrug resistance in a human small cell lung cancer cell line selected in adriamycin. Cancer Research, 47, 2594-2598. 

In 1997, Chakrabarty et al. reported the isolation of 9-carbethoxy-3-methylcarba-zole (5) and 9-formyl-3-methylcarbazole (6) from the roots of M. koenigii (17). These metabolites are the first 9-formyl and 9-carbethoxy carbazole derivatives obtained from plant sources. 9-Formyl-3-methylcarbazole (6) showed weak cytotoxicity against both mouse melanoma B16 and adriamycin-resistant P388 mouse leukemia cell lines. The structural assignment of these two alkaloids was based on the IR- and H-NMR spectra which were lacking any signal of an NH group. Additional structural support for 9-carbethoxy-3-methylcarbazole (5) was provided by the similarity of the UV absorption spectrum with that of a synthetic sample, obtained by reaction of 3-methylcarbazole with ethyl chloroformate in the presence of base. Further structural support for 9-formyl-3-methylcarbazole (6) was derived from a comparison of the UV spectrum and the IR carbonyl absorption (1696 cm ) with those of an authentic sample of 9-formyl-3-methylcarbazole (1700 cm ), prepared by the treatment of 3-methylcarbazole (2) with 98% formic acid (17) (Scheme 2.3). 

The intrinsic plasma membrane fluidity of a series of sarcoma cell lines with various degrees of adriamycin resistance was studied by ESR [91]. The results document a systematic increase in the order parameter, S, as the cells become more and more resistant. At the same time, a parallel increase in the sphingomyelin content was ob-... 

Drummond, J. T., Anthoney, A., Brown, R., and Modrich, P. (1996) Cisplatin and adriamycin resistance are associated with MutLalpha and mismatch repair deficiency in an ovarian tumor cell line. J. Biol. Chem. 16, 19,645-19,648. 

An approach to enhance Pgp inhibition was applied by Matsuo et al. (90). They used liposomes with a covalently bound monoclonal antibody against an extracellular epitope of Pgp (MRK-16). The binding of these liposomes to K-562/ADM cells (adriamycin-resistant human myelogenous leukemia cell line) was higher than that of IgG2A-modified liposomes and liposomes without modification. In addition, when vincristin was encapsulated in all types of liposomes, it was demonstrated that the cytotoxicity of MRK-16-modified liposomes was higher than that of IgG2a and nonmodified liposomes. 

Scambia, G., Ranelletti, F.O., Panici, P.B., De Vincenzo, R., Bonanno, G, Ferrandina, G., Piantelli, M., Bussa, S., Rumi, C., Cianfnglia, M. and Mancuso, S. (1994) Quercetin potentiates the effect of adriamycin in a multidrug resistant MCF-7 human breast cancer cell line P-glycoprotein as a possible target. Cancer Chemother. Pharmacol. 34, 459 -64. 

Minko, T., Kopeckova, P., Pozharov, V. and Kopecek, J. (1998) HPMA copolymer bound adriamycin overcomes MDRl gene encoded resistance in human ovarian carcinoma cell line. J. Controlled Rel. 54 223-233. 

Minko T, Kopeckova P, Kopecek J. Chronic exposure to HPMA copolymer-bound adriamycin does not induce multidrug resistance in a human ovarian carcinoma cell line. J Cont Rel 1999 59 133-148. 

An adriamycin-resistant MCF-7 human breast tumour cell line showed a developed tolerance to... 

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