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Adamantyl 1,2 dioxetane phosphate

Replacement of this colorimetric reaction with a chemiluminescent process provides for better sensitivity as well as easier reusability of membranes. The development of compounds, such as adamantyl 1,2 dioxetane phosphate (AMPPD) (1) provides an easy alternative to previous detection schemes as this compound is also destabilized by alkaline phosphatase, resulting in the production of light, which will expose a standard X-ray film. Probes produced by exactly the same methods as for NBT detection are perfectly usable in this process. The membranes with the bound alkaline phosphatase are soaked in a... [Pg.107]

FIGURE 5.2 Dioxetane chemiluminescence reaction pathway. A, AMPPD adamantyl 1,2-dioxetane phosphate) when X = H or CSPD when X = Cl B, dioxetane anion C, adamantanone D, methyl-oxybenzoate anion and E, light emission at 477 nm. [Pg.313]

Albrecht, S., Ehle, H., Schollberg, K., Bublitz, R., and Horn, A., Chemiluminescent enzyme immunoassay of human growth hormone based on adamantyl dioxetane phenyl phosphate substrate. In Bioluminescence and Chemiluminescence Current Status (P. E. Stanley and L. J. Kricka, eds.), pp. 115-118. Wiley, Chichester, 1991. [Pg.161]

T9, Thorpe, G. H. G., Bronstein, 1., Kricka, L. J., Edwards, B., and Voyta, J. C., Chemiluminescent enzyme immunoassay of alpha-fetoprotein based on an adamantyl dioxetane phenyl phosphate substrate. Clin. Chem. (Winston-Salem, N.C. 35, 2319-2321 (1989). [Pg.179]

In analogy to FIA, CLIAs have also been developed using chemiluminogenic substrates. Dioxetane derivatives such as adamantyl-l,2-dioxetane phosphate are widely used substrates, which exhibit chemiluminescence upon cleavage by alkaline phosphatase. [Pg.2181]

Chemiluminescence and bioluminescence are also used in immunoassays to detect conventional enzyme labels (eg, alkaline phosphatase, P-galactosidase, glucose oxidase, glucose 6-phosphate dehydrogenase, horseradish peroxidase, microperoxidase, xanthine oxidase). The enhanced chemiluminescence assay for horseradish peroxidase (luminol-peroxide-4-iodophenol detection reagent) and various chemiluminescence adamantyl 1,2-dioxetane aryl phosphate substrates, eg, (11) and (15) for alkaline phosphatase labels are in routine use in immunoassay analyzers and in Western blotting kits (261—266). [Pg.275]

Chemiluminescence is the production of light from a chemical reaction. The emitted light is detected with either a luminometer or on photographic film. There are several substrates capable of producing these light emissions and luminol (5-amino-2, 3-dihydro-l, 4-phthalazinedione) and adamantyl 1,2-dioxetane aryl phosphate are two of the most popular.114,115 The former is used with horseradish peroxidase (HRP) coupled antibodies and the latter is used with alkaline phosphatase (AP)-coupled antibodies. Using an AP-cou-pled antibody and adamantyl 1, 2-dioxetane aryl phosphate, Bronstein et al.U5 were able to detect as little as 125 pg of protein in a Western blot. [Pg.53]

Various 1,2-dioxetane derivatives have been synthesized and reported. Among them, AMPPD (Adamantyl Methoxy Phenyl Phosphoryl Dioxetane) is a well known dioxetane bearing a spiroadamantyl group at the 3-position and a phenol phosphate at the 4-position, which is now used for chemiluminescence enzyme immunoassay (CLEIA) using alkaline phosphatase with highly sensitive detection. [Pg.483]

Fig. 41. Alkaline-phosphatase-triggered cleavage of the highly stable adamantyl meta-phenyl phosphate dioxetane, to give the unstable, light-emitting phenoxide ion. The half-life values were measured in an aqueous medium at 30°C (B18,E1). Fig. 41. Alkaline-phosphatase-triggered cleavage of the highly stable adamantyl meta-phenyl phosphate dioxetane, to give the unstable, light-emitting phenoxide ion. The half-life values were measured in an aqueous medium at 30°C (B18,E1).

See other pages where Adamantyl 1,2 dioxetane phosphate is mentioned: [Pg.518]    [Pg.2067]    [Pg.1342]    [Pg.152]    [Pg.429]    [Pg.408]    [Pg.518]    [Pg.312]    [Pg.479]    [Pg.491]    [Pg.10]    [Pg.216]    [Pg.479]    [Pg.491]    [Pg.354]    [Pg.198]    [Pg.234]    [Pg.62]    [Pg.487]    [Pg.149]    [Pg.540]   
See also in sourсe #XX -- [ Pg.1342 ]




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1,2-Dioxetans

1- adamantyl

1.2- Dioxetane

1.2- dioxetan

3- 1,2 dioxetane phosphate

Adamantyl 1,2 dioxetane phosphate AMPPD)

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