Adamantyl 1,2 dioxetane phosphate AMPPD

Replacement of this colorimetric reaction with a chemiluminescent process provides for better sensitivity as well as easier reusability of membranes. The development of compounds, such as adamantyl 1,2 dioxetane phosphate (AMPPD) (1) provides an easy alternative to previous detection schemes as this compound is also destabilized by alkaline phosphatase, resulting in the production of light, which will expose a standard X-ray film. Probes produced by exactly the same methods as for NBT detection are perfectly usable in this process. The membranes with the bound alkaline phosphatase are soaked in a... 

FIGURE 5.2 Dioxetane chemiluminescence reaction pathway. A, AMPPD adamantyl 1,2-dioxetane phosphate) when X = H or CSPD when X = Cl B, dioxetane anion C, adamantanone D, methyl-oxybenzoate anion and E, light emission at 477 nm. 

Various 1,2-dioxetane derivatives have been synthesized and reported. Among them, AMPPD (Adamantyl Methoxy Phenyl Phosphoryl Dioxetane) is a well known dioxetane bearing a spiroadamantyl group at the 3-position and a phenol phosphate at the 4-position, which is now used for chemiluminescence enzyme immunoassay (CLEIA) using alkaline phosphatase with highly sensitive detection. 

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