Acridine orange stain with ethidium bromide

Clumps of suspension-adapted CHO cells stained with ethidium bromide and acridine orange, observed under a fluorescence microscope. Photo taken by Dr. Rodrigo C. V. Pinto, Cell Culture Engnieering Laboratory, Federal University of Rio de Janeiro, Brazil. 

Panel (d) shows magnification of proliferated cells with extended filopodia. Ethidium bromide/acridine orange combined stain causes living cells to fluoresce green whereas apoptotic (dead) cells cause a distinctive red-orange fluorescence (Amaravathy et al., 2014). ( With permission by Elsevier.)... 

SYBR dyes for sensitive detection in gels and blots, Chemically reactive SYBR dyes for bioconjugates. The three classes of classic nucleic acid stains are Intercalating dyes (ethidium bromide and propidium iodide), Minor-groove binders, (DAPI and the Hoechst dyes ). Miscellaneous nucleic acid stains with special properties (acridine orange, 7-AAD and hydroxystilbamidine). 39... 

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