A-Acetyl-p-D-glucosaminidase

Fitz-Gerald, C.H., Deeth, H.C., Kitchen, B.J. 1981. The relationship between the levels of free fatty acids, lipoprotein lipase, carboxylesterase, A-acetyl-p-D-glucosaminidase, somatic cell count and other mastitis indices in bovine milk. J. Dairy Res. 48, 253-265. 

Aoyama, T, Kojima, F., Imada, C. et al (1995) Pyrostatins A and B, new inhibitors of A-acetyl-P-D-glucosaminidase, produced by Streptomyces sp. SA-3501. Journal of Enzyme Inhibition and Medicinal Chemistry, 8, 223-232. 

Studies on the conformational and stereochemical aspects of the Ferrier reaction leading to cyclitols and azido cyclitols as an extension of earlier work (Vol. 19, p. 173) have been reported. The synthesis of racemic 6-acetamido-l,2-anhydro-6-deoxy-ffiyo-inositol as a tight binding inhibitor and pseudosubstrate for A/ -acetyl-p-D-glucosaminidase has appeared starting from racemic tetra-O-acetylconduritol. ... 

Syntheses of amino-sugar glycosides and disaccharides, including chromogenic substrates for A -acetyl-p-D-glucosaminidase, are covered in Chapter 3. Synthesis of protected 2-amino-2-deoxy-glycosyl azides is covered in Chapter 10. 2,3,6-Trideoxy-5-0-(4-nitrobenzoyl)-3-trifluoroacetamido-L-riho-hexofuranosyl bromide, a furanoid ristosamine derivative, was synthesized from L-rhamnal via intermediates reported previously (Vol.25, p.253) to effect glycosylations. ... 

C. T. Yuen, R. G. Price, P. F. G. Praill, and A. C. Richardson, A new and simple colorimetric assay procedure for IV-acetyl-P-D-glucosaminidase in urine,/. Clin. Chem. Biochem., 19 (1981) 884. (Abstr. XI Int. Congr. Clin. Chem.). 

I. Pocsi, S. A. Taylor, A. C. Richardson, K. H. Aamlid, B. V. Smith, and R. G. Price, VRA-GlcNAc Novel substrate for N-acetyl-P-D-glucosaminidase applied to assay of this enzyme in human urine, Clin. Chem., 36 (1990) 1884-1888. 

In male Fischer 344 rats treated intraperitoneally with 0, 10, 20, 40, 60 or 80 mg/kg bw acrylonitrile, significant increases in urinary volume and glucose were observed 24 h after treatment with 20 mg/kg bw (Rouisse et al., 1986). Increased levels of urinary N-acetyl-P-D-glucosaminidase were detected after treatment with 60 mg/kg bw acrylonitrile. Symptoms of nephrotoxicity were also observ ed after a 4-h exposure to 200 ppm [434 mg/m3] acrylonitrile. Histopathological examination revealed lesions in the proximal tubular region of the kidney. 

Renal Effects. Occupational exposure to silver metal dust has been associated with increased excretion of a particular renal enzyme (N-acetyl-p-D glucosaminidase), and with decreased creatinine clearance (Rosenman et al. 1987). Both of these effects are diagnostic of marginally impaired renal function. However, the workers in this study were also exposed to cadmium, which was detected in the urine of 5 of the 27 workers studied. Cadmium is known to be nephrotoxic differentiation of the effects of the two metals in the kidney is not possible with the data presented. Therefore, no conclusion can be drawn regarding renal effects of silver based on this study. 

Occupational exposure to chromium(III) or chromium(O) does not appear to be associated with renal effects. No renal impairment based on urinary albumin, retinol binding protein, and renal tubular antigens was found in 236 workers employed in the ferrochromium production industry where ferrochromite is reduced with coke, bauxite, and quartzite. The mean airborne concentration of chromium in various sample locations was 0.075 mg chromium(III)/m3 chromium(VI) was below the detection limit of 0.001 mg chromium(VI)/m3 at all locations (Foa et al. 1988). Workers employed in an alloy steel plant with a mean exposure of 7 years to metallic chromium at 0.61 mg chromium(0)/m3 and to other metals had normal urinary levels of total protein and p2-microglobulin, enzyme activities of alanine-aminopeptidase, N-acetyl-P-D-glucosaminidase, gammaglutamyl-transpeptidase, and P-galactosi-dase (Triebig et al. 1987). In boilermakers exposed to chromium(O), no increase in urinary levels of... 

Piperidines P-glucosidases and a-glucosidases (including sucrase, maltase, isomaltase and amylase), P-galactosidases and i -acetyl-P-D-glucosaminidases " ... 

In LLC-PKj cells gentamicin induces membrane damage as shown by the loss of specific membrane enzymes (y-glutamyl transpeptidase, alkaline phosphatase and aminopeptidase), a decrease of the lysosomal enzyme N-acetyl-P-D-glucosaminidase, an inhibition of apical Na -dependent glucose transporter and the basolateral Na-K-ATPase pump as well as a decrease in dome formation [141, 142]. Furthermore gentamicin results in a dose dependent decrease in intracellular ATP and cAMP [142]. 

Sugihira N, Saito H. Urinary excretion of N-acetyl-p-D-glucosaminidase and Pj-microglobulinin people living in a cadmium-polluted area. Jpn J Hyg 1986 41 665-671. 

N-acetyl-p-D-glucosaminidase (Figure 4). No significant correlation was evident for other renal parameters U-albumin, U-orosomucoid, U-p2-microglobulin, U-copper, S-creatinine, and S-p2-microglobulin. Studies on chlor-alkali workers in Scandinavia [122-124] have reported minimal and apparently reversible renal effects from mercury exposures in this occupational group as evaluated by urinary excrebon of NAG, albumin and titers of autoantibodies. These investigators noted that a small number of susceptible individuals may exist and that selenium status appears to have a major effect on urinary NAG excretion [124]. 

Bazzi C, Petr ini C, Rizza V, Arrigo G, Napodano P, PapareUa M, et al. Urinary N-acetyl-p-D-glucosaminidase excretion as a marker of tubular cell dysfunction and a predictor of outcome in primary glomerulonephritis. Nephrol Dial Transplant 2002 17 1890-6. 

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