YMC-Pack

General Experimental Procedures MPLC fractionation was performed using an Isco CombiFlash, and HPLC isolation was performed using Shimadzu pumps and detector and YMC-Pack ODS-AQ C18 column. 

Figure 2 HPLC Profile of Synthetic Midkine Intermediates in the Deprotection and Folding Process, (a) Crude Product Obtained by the HF Deprotection Reaction, (b) Purified Peptide (10 Acm). (c) Completely Depro-tected Peptide, (d) After Folding Reaction, (e) Finally Purified Product. Column YMC-Pack ODS A-302 (4.6 x 150 mm) Eluent 10 10% MeCN/0.1% TFA, 25 min at 40 °C Flow Rate 1.0 mL-min-1 Detection 220 nm. Individual Chromatographs from this Figure have Previously Appeared in RefI61...

Oxalic, quinic, tartaric, malic, isocitric, ascorbic, citric, fumaric, succinic, propionic, cis-aconitic Orange juice YMC-Pack ODS-AQ (250 X 4.6 mm) 5 /ttm 20 mM KH2P04, pH 2.8 UV, 214 nm 116... 

Kanazawa et al. [124] determined omeprazole and its metabolites in human plasma by liquid chromatography-three-dimensional quadrupole mass spectrometry with a sonic spray ionization interface. The analytical column was YMC-Pack Pro Cig (5 cm x 2 mm) using acetonitrile-50 mM ammonium acetate (pH 7.25) (1 4) at a flow-rate of 0.2 ml/min. The drift voltage was 30 V. The sampling aperture was heated at 110 °C and shield temperature was 230 °C. In the mass spectrum, the molecular ions of omeprazole, hydroxyomeprazole, and omeprazole sulfone were clearly observed as base peaks. The method is sufficiently sensitive and accurate for pharmacokinetic studies of omeprazole. 

Procedure (See Chromatography, Appendix BA.) Use a suitable high-performance liquid chromatograph equipped with differential refractometer, autosampler injection unit, mobile-phase degasser, column heating block or oven, and a computing integrator. The column is Lichrosorb RP-18 250-mm x 4.5 mm (id) (GL Science, Inc., or equivalent) and YMC-Pack ODA-A A-303 250-mm x 4.5 mm (id) (YMC Company, Ltd., or equivalent) connected in a series, or equivalent, maintained at 50°. Use 80 20 acetone acetonitrile as the eluent, at a flow rate of 2 mL/min. 

Figure 14-2. Normal-phase separation of a mesylate from corresponding acid. Chromatographic conditions YMC Pack Diol 150 x 4.6 mm, 90% 0.1% TFA in heptane/10% 0.1% TFA in THE...

When using BrMAC, postcolumn hydrolysis of separated PG esters is neccessary for on-line detection of the fluorophore, resulting in detection limits of about 10 fmol. Hydrazone or amide derivatives are also suitable for fluorescence detection as well as 3-bro-momethyl-6,7-dimethoxy-l-methyl-2(l//) quinoxali-none (YMC Pack C8) or 9-anthroyldiazomethane (ADAM) characterized by its low stability (Nucleosil ODS silica). Electrochemical detection of active com-... 

Figure 2.20 Three possibilities to improve resolution. Problem separation of acetophenone (first peak) and veratrole. Original conditions column, 5 cm x 2 mm i.d. stationary phase, YMC-Pack ODS-AQ, 3gm mobile phase, 0.3 ml min water-acetonitrile (80 20) UV detector 254 nm. Arwas increased by using water-acetonitrile (85 15), all other conditions being unaltered, a was increased by using water-methanol (70 30). Afwas increased by using a column of 15 cm length under original conditions.

Guard column 5 p-m cyano guard column (YMC) Column 250 X 4.6 5 p,m YMC-Pack cyano (YMC)... 

LC columns were used to measure the simple sugars, glycerol, and ethanol. For monosaccharides, a YMC-Pack Polyaminell coluirm (4.6x250 mm Japan) with column... 

LC Analyses, LC analyses were performed by using a Waters 600 pump equipped with a Rheodyne Model 7725i manual injector (50 pL loop) and a Waters Model 991 photodiode array detection system. Chromatographic separation was achieved on a YMC Pack PolymerCtS (YMC Inc. Wilmington, NC) column with a OPTI-SOLV mini filter (Optimise Technologies Inc., Portland, OR). The linear gradient was 60% H2O (solvent A)/40% acetonitrile (solvent B) which was held for 10 min, then increased by 8% acetonitrile/min to 20% H2O/80% acetonitrile, and held for 15 min. The flow rate was 0.8 mL/min. 

Fig. 1 Reaction of Ehnmb-containmg labeled peptide 14 in a TFA solution (14% CDCI3, 0.5% TCEP HC1 (v/v/w)). (a-c) NMR spectra after indicated reaction time, (d-f) RP-HPLC. Column YMC-Pack Pro C18 (4 6 x 150 nun) eluent 0.1% TFA in aq CH3CN, 1.0 mL/min...

Fig. 3 RP-HPLC profile of the crude BPTI(l-29)-SCH2CH2SO3H (33) prepared via an on-resin N-S acyl shift reaction and transthioesterification. Column YMC-Pack Pro C18 RS (4.6 X 150 mm) eluent 0.1% TFA in aq CH3CN, l.OmL/min.

Fig. 5 RP-HPLC of (a) reaction mixture of the thioesterification of CPE peptide 74, (b) reaction mixture of the ligation of CPE peptide 75 and cysteinyl peptide 76, (c) final reaction mixture after removal of the protecting groups. Column YMC-Pack ProClS (4.6 x 150 mm) (a) or YMC-Pack C8 (4.6 X 150 mm) (b, c), eluent aq CH3CN containing 0.1% TEA, flow rate 1.0 mL/min. 80 H3 (44-135).

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