Yeast extract agar

Not recognizing the need for special media or isolation techniques (eg, charcoal yeast extract agar for isolation of legionella species, shell-vial tissue culture system for rapid isolation of cytomegalovirus)... 

A spore suspension was prepared with 5 ml of a 0.9% sodium chloride solution obtained from a 7-10 day old, malt extract-yeast extract agar slant culture of Mortierella maculata nov. spec. E-97 [NCAIM(P)F 001266] strain able to 6-p-hydroxylate compactin and the suspension was used to inoculate 100 ml inoculum medium PI (glucose-50 g, soybean meal-20 g, in 1000 ml tap water) sterilized in a 500 ml Erlenmeyer flask. 

Table VII. Enumeration of Groundwater Bacteria by Pour Plate Counts on Groundwater-Yeast Extract Agar...

Figure 1 Colonies of microorganisms on agar plates after incubation (A) close-up of bacteria pour plate, plate count agar (B) close-up of mold surface plate, oxytetracycline glucose yeast extract agar (C) Salmonella species streak plate, xylose lysine decarboxylase agar.

Cells are gram negative, rod shaped, measuring 0.4-1.0 by 1.0-2.5 pm, and motile with peritrichous flagella. Colonies are smooth, entire, raised, shiny, and light brown, pink, to dark pinkish on glucose/peptone/yeast extract agar. 

Table 5. System 17 Coolant/Piston With Legionella feelii by Isolation on Charcoal Yeast Extract Agar. ...

Take a loop full of the creamy culture off the agar plate. Dip the loop into a 250 ml flask containing 100ml of 5.0g-l 1 glucose and 1 g-1 1 yeast extract. Swirl the loop in the nutrient solution to dislodge the selected culture from the loop. 

Rps. palustris 420L, entrapped into agar gel, malate,glutamate, yeast extract, over 30 days of 1.2 40 0.0178 44 Vincenzini et al., 1981... 

Variations for 5-hydroxy fluvastatin. Medium for growth on agar plates Plate Count Agar (Fluka/ Sigma Aldrich, Buchs, Switzerland) medium for preculture and main culture glucose 20 g L , soytone (Becton Dickinson) 15 g L , yeast extract 10 g L , pH adjusted to 6.5 with NaOH. Incubation time of main culture before fluvastatin addition 3 days biotransformation period 24 h. 

YEPGA = Yeast extract, peptone and glucose agar. ... 

Materials for media (Bacto Tryptone, Bacto Yeast Extract and Difco SOB Medium, agar powder and NaCl) and antibiotics (ampicillin sodium and kanamycin sulfate). 

Luria-Bertani (LB, 1.0% polypeptone, 0.5% yeast extract, 1.0% NaCl, pH 7.0) medium containing 100 pg/mL ampicillin (LB-amp) and LB-amp agar medium (LB containing... 

Rogosa-type medium (60) made as follows 2% Tryptone (Difco), 0.5% yeast extract (Difco), 0.5% peptone (Difco), 0.5% glucose, 0.005% Tween 80 (Nutritional Biochemicals Corp.), and 2% agar (Difco) in a filtered or centrifuged fourfold dilution (with water) of tomato juice (containing no preservatives). The medium is adjusted to pH 5.5 with HC1 before adding agar. 

Natural media are those used on the basis of experience and not on the basis of exact knowledge of their composition and action. Natural or complex media usually contain peptones, beef extract, or yeast extract. When a solid medium is desired, a solidifying agent such as gelatin or agar may be incorporated into the medium. Examples of a relatively simple liquid and a solid medium that support the growth of many common heterotrophs are nutrient broth and nutrient agar. Their composition is as follows ... 

Prepare 1% yeast extract (Saccharomyces cerevisae), 1% peptone, 2% glucose and 1% agar (by weight). 

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