Yeast chromosomal translocation

PFG can vastly simplify genetic analysis as demonstrated in its application to analysis of yeast chromosomal translocations. 

Figure 5. Yeast Chromosomal Trsmslocation Analysis. Ethidium bromide-stained gel. The first lane was loaded with size markers eus in Figure 4. Other lanes were alternately loaded with wild-type yeast (no translocations) and a strain containing a translocation between chromosomes 3 and 12. Experimental conditions were as in Figure 3, using 1.5% low endoosmosis agarose gel. The arrow indicates the position of the wild-type chromosome and the absence of the translocated chromosome.

Epoxybutane induced morphological transformation, sister chromatid exchanges, chromosomal aberrations and mutation in cultured animal cells however, in a single study, it did not induce unscheduled DNA synthesis in rat primary hepatocytes. It induced sex-linked recessive lethal mutations and translocations in Drosophila melanogaster, mitotic recombination in yeast, and mutations in yeast and fungi. 1,2-Epoxybutane induced DNA damage and mutations in bacteria. 

P-Propiolactone was mutagenic to bacteria. In yeast, it induced mitotic gene conversion, aneuploidy and mutations. It produced heritable translocations and sex-linked recessive lethal mutations in Drosophila melanogaster. In vitro, it induced cell transformation and gene mutations in human cells, and cell transformation, gene mutations, chromosomal aberrations and sister chromatid exchanges in mammalian cells. 

As previously summarized, diethyl sulfate induced mutation and DNA damage in bacteria and induced reverse mutation and mitotic recombination in yeast. In plant cells, diethyl sulfate induced chromosomal aberrations. In a single study, diethyl sulfate did not induce heritable translocation in Drosophila melanogaster but did induce autosomal recessive lethal mutations, sex-linked recessive lethal mutations and genetic crossing-over. In cultured mammalian cells, diethyl sulfate induced chromosomal aberrations, mieronueleus formation, sister chromatid exchanges, forward mutation and DNA singlestrand breaks it also induced unscheduled DNA synthesis in primary cultures of rat... 

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