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X-gal

Procedures of the beta-galactosidase activity measuring using colour reaction with ONPG and X-Gal without cells permeabilization were developed and the detection limit at the level of 4 ppb has been achieved. The influence of the foreign ions (phosphate, sulphate, carbonate et. al) was studied. [Pg.428]

FIGURE 13.19 The structure of 5-bromo-4-chloro-3-iudolyl-/3-D-galactopyra-uoside, or X-gal. [Pg.416]

T. A. Gerken, Kinetic modeling confirms the biosynthesis of mucin core 1 (/i-Gal (1 -3)x-Gal YAc-O-Ser/Thr) O-glycan structures are modulated by neighboring glycosylation effects, Biochemistry, 43 (2004) 4137—4142. [Pg.162]

Cells will grow on agar plates containing Ampicillin and X-gal and colonies will be blue in color... [Pg.50]

Bacteria containing the pUC8 plasmid with a gene inserted into the LacZ gene grown on medium containing X-gal are blue in colour BECAUSE... [Pg.473]

Plate on 24-well format LB agar supplemented with antibiotic and, if appropriate, X-Gal and IPTG (dilute a 20% X-Gal, in dimethyl formamide, stock 1 1000, dilute the IPTG 500 mM stock 1 500 in warm agar before pouring). Plate 10 pi of cells, shake plates well to spread the cell suspension, and allow at least 10-15 min for the plates... [Pg.28]

Operator site / now allows gene expression 1 Makes ArsR protein, Makes A. p-galactosidase V i X-Gal (colorless)... [Pg.1]

Figure 21.2 Histochemical analysis of / -galactosidase gene expression in liver. Mice were injected with 1.6ml saline containing various amounts of pCMV-LacZ plasmid DNA. Animals were sacrificed eight hours post injection and liver sections were made using cryostat. Sections (A, B, C and D) were stained with X-gal solution followed by eosin for counter-stain. Sections (E, F, G and H) were stained by a standard hematoxylin/eosin staining method. Sections were made from animals each receiving 0 (A, E), 0.5 (B, F), 2.5 (C, G) and 25 pg (D, H) of pCMV-LacZ. (25x). (see Color Plate 14)... Figure 21.2 Histochemical analysis of / -galactosidase gene expression in liver. Mice were injected with 1.6ml saline containing various amounts of pCMV-LacZ plasmid DNA. Animals were sacrificed eight hours post injection and liver sections were made using cryostat. Sections (A, B, C and D) were stained with X-gal solution followed by eosin for counter-stain. Sections (E, F, G and H) were stained by a standard hematoxylin/eosin staining method. Sections were made from animals each receiving 0 (A, E), 0.5 (B, F), 2.5 (C, G) and 25 pg (D, H) of pCMV-LacZ. (25x). (see Color Plate 14)...
Ligated plasmids should be checked for insertion of the desired fragment instead of just religation, most conveniently by blue-white screening. In vectors with the lacZ gene sequence, insertion at any restriction site of the MCS results in disruption of that sequence so that no /i-galactosidase is expressed and the synthetic X-gal substrate cannot form blue colonies. [Pg.62]

TK Tn-368 Tn 7 tPA tRNA UTR X-gal YACs virus thymidine kinase cell line originated from the ovary of the insect Trichoplmia ni transposon 7 tissue plasminogen activator transfer ribonucleic acid untranslated regions 5-bromo-4-chloro-3-indolyl- -D-galactoside yeast artificial chromosomes... [Pg.537]

See other pages where X-gal is mentioned: [Pg.252]    [Pg.1076]    [Pg.230]    [Pg.68]    [Pg.491]    [Pg.416]    [Pg.117]    [Pg.48]    [Pg.71]    [Pg.68]    [Pg.50]    [Pg.39]    [Pg.468]    [Pg.473]    [Pg.88]    [Pg.88]    [Pg.248]    [Pg.1]    [Pg.429]    [Pg.230]    [Pg.1494]    [Pg.904]    [Pg.904]    [Pg.904]    [Pg.157]    [Pg.426]    [Pg.452]    [Pg.79]    [Pg.345]    [Pg.137]    [Pg.254]    [Pg.267]    [Pg.69]    [Pg.36]    [Pg.578]    [Pg.174]    [Pg.64]    [Pg.68]   
See also in sourсe #XX -- [ Pg.254 , Pg.267 ]

See also in sourсe #XX -- [ Pg.271 ]

See also in sourсe #XX -- [ Pg.203 , Pg.204 , Pg.207 ]

See also in sourсe #XX -- [ Pg.254 , Pg.267 ]



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