Vitro Characterization

Stevens PW, Raffen R, Hanson DK, Deng YL, Berrios Hammond M, Westholm FA, Murphy C, Eulitz M, Wetzel R, Solomon A, et al. Recombinant immunoglobulin variable domains generated from synthetic genes provide a system for in vitro characterization of light-chain amyloid proteins. Protein Sci 1995 4 421-432. 

Ruch, S., P. Beyer et al. (2005). Retinal biosynthesis in eubacteria In vitro characterization of a novel carotenoid oxygenase from Synechocystis sp. PCC 6803. Mol. Microbiol. 55(4) 1015-1024. 

Scherzinger, D., S. Ruch et al. (2006). Retinal is formed from apo-carotenoids in Nostoc sp PCC7120 in vitro characterization of an apo-carotenoid oxygenase. Biochem. J. 398 361-369. 

Varady, M., Bjorn, H., Craven, J. and Nansen, P. (1997) In vitro characterization of lines of Oesophagostomum dentatum selected or not selected for resistance to pyrantel, levamisole and ivermectin. International Journal for Parasitology 27, 77-81. 

The evidence for flavin involvement as deduced from action, absorption, and fluorescence spectra, as well as via non-spectroscopic methods, is then evaluated. It is concluded that all experimental results indirectly support the flavin hypothesis, but that direct proof will have to await the isolation and in vitro characterization of the chromophore. 

Neu M, Germershaus O, Mao S, Voigt KH, Behe M, Kissel T (2007) Crosslinked nanocarriers based upon poly(ethylene imine) for systemic plasmid delivery in vitro characterization and in vivo studies in mice. J Control Release 118 370-380... 

Coffield, J. A., Bakry, N., Zhang, R. D., Carlson, J., Gomella, L. G. and Simpson, L. L. In vitro characterization of botuli-num toxin types A, C and D action on human tissues combined electrophysiologic, pharmacologic and molecular biologic approaches. J. Pharmacol. Exp. Ther. 280 1489-1498,1997. 

Pauli J, Vag T, Haag R, Spieles M, Wenzel M, Kaiser WA, Resch-Genger U, Hilger I (2009) An in vitro characterization study of new near infrared dyes for molecular imaging. Eur J Med Chem 44 3496-3503... 

Wheatley MA, Dent J, Wheeldon E B Smith P L (1988) Nasal drug delivery An in vitro characterization of transepithelial electrical properties and fluxes in the presence or absence of enhancers. J Control Release 8 167-177. 

In this chapter, we will review studies on formulation variables affecting monocyte and macrophage targeting (e.g., size and number of vesicles), in vitro characterization in cell cultures, and in vivo immunomodulation and anti-inflammatory responses. 

In Vitro Characterization and Optimization of the Liposomal Vaccine System by FACS Analysis... 

Beher, D., Flicker, M., Nadin, A., et al. (2003) In vitro characterization of the presenilin-dependent gamma-secretase complex using a novel affinity ligand. Biochemistry, 42, 8133-8142. 

Meier E, Frederiksen K, Nielsen M, Pharmacological in vitro characterization of the arecoline bioisostere Lu 25-109-T, a muscarinic compound with M-l-agonistic and M-2/M-3 antagonistic properties. Drug Dev Res 40 1—16, 1997. 

Although genetic studies have identified a number of putative aromatic PK aromatases and/or cyclases, in vitro characterization of these enzymes is usually difficult since the postulated Hnear or monocyclic poly 3-ketone templates are often unstable to be isolated or synthesized. For these reasons, the generality and synthetic applications of these enzymes have not been studied in most cases. However, there is plenty of evidence to suggest that enzymatic aromatization and cyclization take place for a variety of linear precursors with various chain lengths [32]. 

Menendez A, Laidlaw J, Raventos-Suarez C, et al. In vitro characterization of BMS-184476, a novel taxane with promising preclinical activity. Proc Am Assoc Cancer Res 2000 41 554 (abstract 3529). 

P. Johnstrdm, J.H. Rudd, H.K. Richards, T.D. Fryer, J.C. Clark, P.L. Weissberg, J.D. Pickard, A.P. Davenport, Imaging endothelin ETb receptors using [ F]-BQ3020 In vitro characterization and positron emission tomography (microPET), Exp. Biol. Med. (Maywood) 231 (2006) 736-740. 

B. D. Weintraub, M. S. Szkunlinski (1999). Development and in vitro characterization of human recombinant thyrotropin. Thyroid 9 447. 

Hamdan-Khalil R, Allorge D, Lo-Guidice JM et al. In vitro characterization of four novel non-functional variants of the thiopurine -methyltransferase. Biochem Biophys Res Commun 2003 309 1005-1010. 

Muller, R.H., Lherm, C., Jaffray, P., and Couvreur, P. (1988). Toxicity of cyanoacrylate particles in L929 fibroblast cell-cultures-relation between toxicity and in vitro characterization parameters. Archiv Der Pharmazie, 321, 681-681. 

Cui, F., Shi, K., Zhang, L., Tao, A., Kawashima, Y. (2006). Biodegradable nanoparticles loaded with insulin-phospholipid complex for oral delivery preparation, in vitro characterization and in vivo evaluation. Journal of Controlled Release, 114, 242-250. 

Bigoni, R., Calo, G., Rizzi, A., Guerrini, R., De Risi, C., Hashimoto, Y., Hashiba, E., Lambert, D.G., Regoli, D. In vitro characterization of J-113397, a non-peptide nociceptin/orphanin FQ receptor antagonist, Naunyn-Schmiedeberg s Arch. Pharmacol. 2000, 361, 565-568. 

McKendree, W.L., Paul, A.-L., DeLisle, A.J. Ferl, R.J. (1990). In vivo and in vitro characterization of protein interactions with the dyad G-box of the Arabidopsis Adh gene. The Plant Cell 2, 207-14. 

Lim, S.T., et al. 2001. In vivo and in vitro characterization of novel microparticulates based on hyaluronan and chitosan hydroglutamate. AAPS Pharmsci Tech 2 article 20. 

Parnigotto, P.P., et al. 1998. Bovine corneal stroma and epithelium reconstructed in vitro. Characterization and response to surfactants. Eye 12 304. 

W. M. Kati, D. Montgomery, R. Carrick, L. Gubareva, C. Maring, K. McDaniel, K. Steffy, A. Molla, F. Hayden, D. Kempf, and W. Kohlbrenner, In vitro characterization of A-315675, a highly potent inhibitor of A and B strain influenza virus neuraminidases and influenza virus replication, Antimicrob. Agents Chemother., 46 (2002) 1014-1021. 

Zhang C, Albermann C, Fu X et al (2006) The in vitro characterization of the iterative avermectin glycosyltransferase AveBI reveals reaction reversibility and sugar nucleotide flexibility. J Am Chem Soc 128 16420-16421... 

Norton LW, Tegnell E, Toporek SS, Reichert WM. In vitro characterization of vascular endothelial growth factor and dexamethasone releasing hydrogels for implantable probe coatings. Biomaterials 2005, 26, 3285-3297. 

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