Viruses neoplastic transformation

Dunkel VC, Pienta RJ, Sivak A, et al. 1981. Comparative neoplastic transformation responses of Balb/3T-3 cells, Syrian hamster embryo cells, and Rauscher murine leukemia virus-infected Fischer 344 rat embryo cells to chemical carcinogens. J Nat Cancer Inst 67 1303-1315. 

Rhim JS, Jay G, Arnstein P, Price FM, Sanford KK, Aaronson SA. Neoplastic transformation of human epidermal keratinocytes by AD12-SV40 and Kirsten sarcoma viruses. Science 1985 227 1250-1252. 

Neoplastic transformation The uncontrollable division of host cells caused by infection with a DNA tumor virus. 

In some cases, two or more O. must be present in a virus for it to be able to transform cells in vitro (especially in the case of primary cells, i.e. cells taken directly from an animal and cultured briefly in vitro) this is usually true in viva Some O. are able by themselves, to induce transformation of certain cell lines in vitra The standard test of transforming ability for DNA is transfection of NIH 3T3 cells (an immortalized line of mouse cells). Some authors argue that these cells have already taken the first step towards neoplasticity, and that the ability of an O. to transform NIH 3T3 cells in vitro does not prove that it can irtdtice a cancer in vivo in the absence of other oncogenic factors. 

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