Vesicular stomatitis virus mutants

Moreau, M.-C. Inhibition of a vesicular stomatitis virus mutant by rifampin. J. Virol. 

Other mutants of Chinese-hamster ovary-cells having decreased amounts of lipid-linked oligosaccharides are known, although they are less well characterized. G-Protein of vesicular stomatitis virus grown in one of these mutants appeared to contain fewer, but full-sized rather than truncated, oligosaccharide side-chains. There may be insufficient amounts of oligosaccharide precursor available for transfer to nascent glycoproteins.172... 

PPIases also play a key role in cell entry and in the replication of several pathogenic viruses, as well as in the formation of mature virions. The vesicular stomatitis virus New Jersey serotype, which causes major diseases in animals, particularly cattle, contains several host proteins, including cyclophilin A, which are essential for viral replication. CsA and overexpression of catalytically inactive mutants of CypA drastically inhibit gene expression of the virus New Jersey serotype but have little effect on the virus Indiana serotype [102]. Although it is presently the subject of controversial publications and is still misunderstood, the involvement of PPIases - notably hCypl8 - in the HIV-1 life cycle is one of the most famous examples of the hijacking of host proteins by a lentivirus [8]. 

In both procedures, a gene encoding an abundant membrane glycoprotein (G protein) from vesicular stomatitis virus (VSV) Is Introduced Into cultured mammalian cells either by transfection or simply by Infecting the cells with the virus. The treated cells, even those that are not specialized for secretion, rapidly synthesize the VSV G protein on the ER like normal cellular secretory proteins. Use of a mutant encoding a temperature-sensitive VSV G protein allows researchers to turn subsequent protein transport on and off. At the restrictive temperature of 40 °C, newly made VSV G protein Is misfolded and therefore retained within the ER by quality control mechanisms discussed in Chapter 16, whereas at the permissive temperature of 32 C, the accumulated... 

A procedure has been described for the selective isolation of mutants of vesicular stomatitis virus, defective in the production of the viral glycoprotein." ... 

ZUberstein, A., Snider, M. D., Porter, M., and Lodish, H. F. (1980). Mutants of vesicular stomatitis virus blocked at different stages in maturation of the viral glycoprotein. Cell 21, 417 27. 

Recent studies have shown that CI-976 produces a potent but reversible block in the export of vesicular stomatitis virus (VSV) G membrane glycoprotein in COPII coated vesicles from the ER (Brown et al, submitted). These studies utilized the well-established thermo-reversible mutant (ts045) of VSV-G to stage the synchronous export of VSV-G from the ER (Bannykh et al., 1998 Fabbri et al, 1994). For these studies, cells were infected with ts045 VSV and incubated at the restrictive temperature for 3.5 h to accumulate VSV-G in the ER (Fig. 2A, B). In solvent-treated control cells shifted to the permissive temperature for 15 min, VSV-G rapidly exited the ER and was transported to the Golgi complex (Fig. 2C, D). In contrast, when CI-976 was included following shift to the permissive... 

Farmilo, A. J., and Stanners, C. P., 1972, Mutant of vesicular stomatitis virus which allows DNA synthesis and division in cells synthesizing viral RNA, J. Virol. 10 605. 

Genty, N., 1975, Analysis of uridine incorporation in chicken embryo cells infected with vesicular stomatitis virus and its temperature-sensitive mutants Uridine transport, J. Virol. 15 8. 

McAllister, P. E., and Wagner, R. R., 1976, Differential inhibition of host protein synthesis in L cells infected with RNA temperature-sensitive mutants of vesicular stomatitis virus, J. Virol. 18 550. 

There is a vast literature on temperature-sensitive and other mutants of vesicular stomatitis viruses, a field pioneered and reviewed in this series by Pringle (1977). The temperature-sensitive ts) mutants... 

Dille, B. J., Hughes, J. V., Johnson, T. C., Rabinowitz, S. G., and Dal Canto, M. C., 1981, Cytopathic effects in mouse neuroblastoma cells during a nonpermissive infection with a mutant of vesicular stomatitis virus, J. Gen. Virol. 55 343. 

Flamand, A., and Bishop, D. H. L., 1973, Primary in vivo transcription of vesicular stomatitis virus and temperature-sensitive mutants of five vesicular stomatitis virus complementation groups, J. Virol. 12 1238. 

Marvaldi, J., and Lucas-Lenard, J., 1977, Differences in the ribosomal protein gel profile after infection of L cells with wild-type or temperature-sensitive mutants of vesicular stomatitis virus. Biochemistry 16 4320. 

Nilsen, T. W., Wood, D. L., and Baglioni, C., 1981, Cross-linking of viral RNA by 4 -aminoethyl-4,5,8-trimethyl-psorate in HeLa cells infected with encephalomy-ocarditis virus and the tsGU4 mutant of vesicular stomatitis virus. Virology 109 82. 

Obijeski, J. F., and Simpson, R. W., 1974, Conditional lethal mutants of vesicular stomatitis virus. II. Synthesis of virus-specific polypeptides in nonpermissive cells infected with RNA host-restricted mutants. Virology 57 369. 

Pringle, C. R., 1978, The tdCE and hrCE phenotype Host range mutants of vesicular stomatitis virus in which polymerase function is affected. Cell 15 597. 

Sekellick, M. J., and Marcus, P. I., 1979, Persistant infection. II. Interferon-inducing temperature-sensitive mutants as mediators of cell spacing. Possible role of persistent infection by vesicular stomatitis virus. Virology 95 36. 

Wertz, G. W., and Levine, M., 1973, RNA synthesis by vesicular stomatitis virus and a small plaque mutant Effects of cycloheximide, J. Virol, 12 253. 

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