Vector-mediated Delivery

Eiedler M, Rodicker E, Salucci V, Lu M, Aurisicchio L, Dahmen U, Jun L, Dirsch O, Putzer BM, Palombo E, Roggendorf M (2004) Helper-dependent adenoviral vector-mediated delivery of woodchuck-specific genes for alpha interferon (IFN-alpha) and IFN-gamma IFN-alpha but not IFN-gamma reduces woodchuck hepatitis virus replication in chronic infection in vivo, J Virol 78 10111-10121... 

Figure 2.9. Differential pharmacological effect elicited by vector-mediated delivery of a VIP analogue. The organ blood flow in brain and salivary gland was measured in conscious rats after i.v. administration of vehicle (saline), the brain delivery vector OX26-SA, the VIP peptide alone, or the chimeric peptide. While cerebral blood flow increased in the chimeric peptide group by 60% compared to the saline control, the increase in salivary gland blood flow seen with the peptide alone was abolished by coupling to the vector. The VIP analogue was biotinylated with a non-cleavable 14-atom spacer (biotin-XX) for coupling to the vector. Data from reference [95].

Pickles RJ. 2004. Physical and biological barriers to viral vector-mediated delivery of genes to the airway epithelium. Proc Am Thorac Soc. 1 302-308. 

Stable intracellular expression of transcriptionally active ribozymes can be achieved by viral vector-mediated delivery. Currently, retroviral vectors are the most commonly used in cell culture, primary cells... 

Pardridge, W.M., Boado, R.J. and Kang, Y.-S. (1995) Vector-mediated delivery of a polyamide ( peptide ) nucleic acid analogue through the blood-brain barrier in vivo. Proc. Natl. Acad. Sci. USA, 92, 5592-5596. 

Carrier- and Vector-Mediated Delivery Systems for Biological... 

Bickel U, Par dr id ge WM. Vector-mediated delivery of opiod peptides to the brain. NIDA Res Monogr 1995 154 28-46. 

Connor B (2001) Adenovual vector-mediated delivery of glial cell line-derived neurotrophic factor provides neuroprotection in the aged parkmsonian rat. Clin Exp Pharmacol Physiol 28 896—900. 

Saito, Y., Buciak, )., Yang, J., and Pardridge, W.M. (1995) Vector-mediated delivery of I-labeled beta-amyloid peptide A beta 1-40 through the blood-brain barrier and binding to Alzheimer disease amyloid of the A beta 1-40/vector complex, Proc Natl Acad Sd USA 92, 10227-10231. 

Lentiviruses can infect non-dividing cells, thereby allowing stable gene transfer in post-mitotic mature neurons. Lentiviral vector-mediated delivery of short hairpin RNAs (shRNAs) results in persistent knockdown of gene expression. In addition, inducible lentiviral vectors offer a powerful tool for better assessing the function of a gene candidate in targeted neurons by an on-off system [1]. 

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