Vault protein

Berger W, Elbling L, Micksche M (2000) Expression of the major vault protein LRP in human non-small-cell lung cancer cells Activation by short-term exposure to antineoplastic drugs. Int J Cancer 88(2) 293-300. 

Scheffer GL, Wijngaard PL, Flens MJ, Izquierdo MA, Slovak ML, Pinedo HM, Meijer CJ, Clevers HC, Scheper RJ (1995) The drug resistance-relateid protein LRP is Ae human major vault protein. Nat Med 1 578-582 Schiemann U, Assert R. Moskopp D, Gellner R, Hengst K, Gullotta F, Domschke W, Pfeiffer A (1997) Analysis of a protein kinase C-alpha mutation in human pituitary tumours. ] Endocrinol 153 131-137... 

In an interesting aside, many years after these synthetic capsules were first prepared, it was found that cells produce protein vaults that have similar encapsulating abilities but on a much larger scale. Whereas cryptands bind single alkali metal cations, vault proteins bind RNA composed of over 140 bases and other similarly sized guests. These biological crypts are discussed later. 

Lactate dehydrogenase Lipoprotein lipase M2 pyruvate kinase MVP (Major vault protein)... 

Slovak, M., Ho, J., Cole, S., Deeley, R Greenberger, F de Vries, E Broxterman, H., Scheffer, G., and Scheper, R. (1995) The FRP gene encoding a major vault protein associated with drug resistance maps proximal to MRP on chromosome 16 evidence that chromosome breakage plays a key role in MRP or FRP gene amplification. Cancer Res. 55,4214-4219. 

Sisodiya, S.M. et al. (2006) Vascular colocalization of P-glycoprotein, multidrug-resistance associated protein 1, breast cancer resistance protein and major vault protein in human epileptogenic pathologies. 

Berger, W. et al. (2001) Overexpression of the human major vault protein in astrocytic brain tumor cells. International Journal of Cancer, 94 (3), 377—382. 

Finally, a protein has been identified that is unique from the previously discussed ABC proteins. This protein, called lung resistance protein or major vault protein, has a molecular weight of 110 kD and is found not in the cell membrane but rather the nuclear membrane or the cytoplasm. The role of this protein is to prevent the drug from accessing the nucleus by trapping the drug in vesicles called vaults. The vaults then shuttle... 

Herrmann C, Golkaramnay E, Inman E et al. Recombinant major vault protein is targeted to neu-ritic tips of PC12 cells. J Cell Biol 1999 144(6) 1163-72. 

Hamill DR, Suprenant KA. Characterization of the sea urchin major vault protein A possible role for vault ribonucleoprotein particles in nucleocytoplasmic transport. Dev Biol 1997 190(1) 117-28. 

Scheffer GL, Wijngaard PL, Flens MJ et al. The drug resistance-related protdn LRP is the human major vault protein. Nat Med 1995 l(6) 578-82. 

Kickhoefer VA, Siva AC, Kedersha NL et al. The 193-kD vault protein, VPARP, is a novel poly(ADP-rihose) polymerase. J Cell Biol 1999 146(5) 917-28. 

Schroeijers AB, Siva AC, Scheffer GL et al. The Mr 193,000 vault protein is up-t< lated in mulddrug-resistant cancer cell lines. Cancer Res 2000 60(4) 1104-10. 

Figure 18.13. Molecular weight measurements of the human major vault protein (MVP). MALDI-TOF-MS (A) and ESl-QTOF-MS (B) of the denatured MVP protein (pH < 3) show the molecular weight of the MVP chain to be 96.8 kDa, (C) ESI-GEMMA of native MVP (pH 6.8) demonstrates the assembly of the MVP vault to a 96-mer, 9.4-MDa complex.

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