Trehalase

Inhibitory Activity (M) of Validamycin and Validoxylamine against Trehalase... 

Compound Trehalase inhibitory activity IC (Af) Dendroid test method MIC (/ig/mL)... 

Among the eight possible diastereoisomers of carba-trehalose of type C, only that isomer having the same configuration as the natural trehalose was found to be a substrate of certain maltases or trehalases. ... 

As insects and certain other organisms utilize a,a-trehalose (a-D-glucopy-ranosyl a-D-glucopyranoside) as their storage carbohydrate, and rely on trehalase to release o-glucose, deoxyfluoro derivatives of trehalose may be... 

Other inverting glucosidases which conform to the pattern of direct hydrolysis of glycosyl fluorides having the correct anomeric configuration, and transglycosylation with inversion if the anomeric configuration is opposite to that of the natural substrates are trehalase from rabbit renal cortex and from the yeast Candida tropicalis, and ) -D-xylosidase from Bacillus pu-milis. ... 

The enzyme was examined by Fischer following earlier studies by Bour-quelot (39) in France. Bourquelot (39) reported that he had made known in 1883 that an extract from a culture of A. niger hydrolyzed maltose. He now provided evidence that the solution also caused hydrolysis of trehalose. However, since this activity was lost on heating to 63 °C but that responsible for the hydrolysis of maltose was maintained up to 75 °C, he concluded that the fungus produced two different ferments —one a maltase, the other a trehalase. 

The chemical synthesis of derivatives and emalogs has long coimnanded much interest, as such compounds are required for the study of structure—activity relationships in the action of trehalases (23,28), may serve as substitutes for 51 in the synthesis of cord-factor analogs to be used as probes in the field of mycobacterial biochemistry (29, 30), and could possibly prove to possess interesting properties as enzyme inhibitors or antibiotic agents. 

Richardson s interest in these trehalose epoxides stemmed from his desire to prepare a wide range of modified trehalose analogues for evaluation as inhibitors of the trehalase of insects, as trehalose is known to be the storage carbohydrate of many insects and good inhibitors of this processing enzyme could potentially function as green insecticides. 

It has been found that both l-thio-a,a-trehalose (94) and a-D-glucopyranosyl 1-thio-a-D-mannopyranoside (95) are inhibitors for cockchafer trehalase [69]. 

Defaye J, Driguez H, Henrissat B, Bar-Guilloux E (1980) Some recent aspects of the specificity and mechanisms of action of trehalases. In Marshall JJ (ed) Mechanisms of saccharide polymerization and depolymerization. Academic Press, New York, p 331... 

Trehalase (from kidney cortex). Purified by solubilising in Triton X-100 and sodium deoxycholate, and submitting to gel filtration, ion-exchange chromatography, conA-Sepharose chromatography, phenyl-Sepharose CL-4B hydrophobic interaction chromatography, Tris-Sepharose 6B affinity and hydrolyapatite chromatography. Activity was increased 3000-fold. [Yoneyama Arch Biochem Biophys 255 168 1987],... 

Besides his fundamental research in the carbohydrate field, the functions of Courtois as the head of a hospital laboratory for many years led him to publish a number of papers dealing with clinical chemistry, among which may be cited determination of ethyl alcohol, proteins, acidic phosphatases, and trehalase in blood determination of the basic groups of proteins by phytic acid study of the phytosoluble glycoproteins in biological fluids and identification and determination of scyllitol in urine. Under the aegis of the International Pharmaceutical Federation, he participated in the standardization of the methods proposed for the assay of such enzymes as cellulases and hemicellulases. 

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