Sepharose-ConA

Trehalase (from kidney cortex). Purified by solubilising in Triton X-100 and sodium deoxycholate, and submitting to gel filtration, ion-exchange chromatography, conA-Sepharose chromatography, phenyl-Sepharose CL-4B hydrophobic interaction chromatography, Tris-Sepharose 6B affinity and hydrolyapatite chromatography. Activity was increased 3000-fold. [Yoneyama Arch Biochem Biophys 255 168 1987],... 

ConA-Sepharose bound weakly bound strongly... 

In the second separation procedure, ConA coupled to Sepharose 2B was added to the vesicle mixture to preferentially bind the sensitized vesicles. After a 10-min incubation on ice, the mixture was filtered and... 

Aryl sulfatase (variant form) Concanavalin A ConA-Sepharose 149... 

Cholesterol ester hydrolase Concanavalin A ConA-Sepharose 155... 

Antigens of Fasciola hepatica Concanavalin A ConA-Sepharose 181... 

Lectin from Phaseolus Concanavalin A ConA-Sepharose 186... 

Human fibroblast interferon Concanavalin A with glutaraldehyde ConA-Sepharose 193... 

Aoyama investigated the inclusion potential of resorcin[4]arenes through encapsulation of fluorescent dyes. In a preliminary study, [71] it was shown that the glucose cluster 4 and galactose cluster 2 (Fig. 22.2) were able to encapsulate eosin Y in a 1 1 manner. Cluster 4 could readily bind to sepharose gel-supported ConA as a model of a surface cell and thus present the drug for uptake. Taking this further. 

An early analysis of the spleen enzyme showed the presence of hexose and hexo-samine A more recent study has demonstrated an enzyme from human hairy leukemic cells which appears identical to the purple spleen phosphatase, and which binds to ConA-Sepharose and is eluted with methylmannoside Very likely, therefore, the spleen enzyme, Uke uteroferrin, is a high mannose glycoprotein, but the structure of its carbohydrate has yet to be determined. In each protein the oligosaccharide chain appears to be attached to an asparagine residue (at position 97 in uteroferrin), since this residue was observable only after treatment with N-glycanase which removes N-asparagine-linked oUgosaccharides. ... 

A purple add phosphatase, substantially similar to uteroferrin and bovine spleen add phosphatase in molecular weight, iron content, and binding to ConA-Sepharose, has been isolated from rat spleen. However, the protein is heterogeneous by isoelectric focusing, perhaps because of variations in carbohydrate content. This protein appears identical to a purple add phosphatase found in developing rat bone and also bears some similarities to a less well diaracterized add phosphatase with protein phosphoty-... 

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