Transplastomic plants

Transplastomic plants Yield, multiple gene expression, low toxicity, containment Absence of glycosylation... 

Leelavathi, S. and Reddy, V.S. (2003). Chloroplast expression of His-tagged GUS fusion a general strategy to overproduce and purify foreign proteins using transplastomic plants as bioreactors. Mol. Breed. 11 49-58. 

In general, expression levels of recombinant proteins in transplastomic plants can be significantly higher than those in nuclear transformants, subject to optimal design of the expression cassette and depending on the nature of the recombinant protein of interest. Clear evidence of an impact of... 

This chapter reviews progress and challenges in the area of production of recombinant proteins, in particular biopharmaceuticals, in plants. Different expression platforms are summarized, including those based on the use of transgenic, transplastomic or transfected plants as production hosts. The quality and yield of recombinant proteins produced in and purified from plants, as well as progress in clinical trials with plant-made pharmaceutical proteins are described. The advantages, limitations and biological safety aspects of plant-based production of biopharmaceuticals are discussed. 

Reddy, V. S., et ah. Analysis of chloroplast transformed tobacco plants with cryllaS under rice psbA transcriptional elements reveal high level expression of Bt toxin without imposing yield penalty and stable inheritance of transplastome. Mol Breed, 2002 9 259-269. 

Yamamoto, H., Kato, H., Shinzaki, Y., Horiguchi, S., Shikanai, T., Hase, T., Endo, T., Nishioka, M., Makino, A., Tomizawa, K., and Miyake, C. (2006). Ferredoxin hmits cyclic electron flow around PSI (CEF-PSI) in higher plants-stimulation of CEF-PSI enhances non-photochemical quenching of Chi fluorescence in transplastomic tobacco. Plant Cell Physiol. 47, 1355-1371. 

Apel, W., and Bock, R. (2009) Enhancement of carotenoid biosynthesis in transplastomic tomatoes by induced lycopene-to-provitamin A conversion. Plant Physiol, 151, 59-66. 

Lossl A, Bohmert K, Harloff H, Eibl C, Muhlbauer S, Koop HU (2005) Inducible trans-activation of plastid transgenes expression of the R. eutropha phb operon in transplastomic tobacco. Plant Cell Physiol 46 1462-1471... 

Lossl, A., Eibl, C., Harloff, H. J., Jung, C., Koop, H. U. Polyester synthesis in transplastomic tobacco (Nicotiana tabacum L.) significant contents of polyhydroxybutyrate are associated with growth reduction. Plant Cell Rep 2003,21, 891-899. 

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