Transformation protocols

Schechtman LM (1985) BALB/c 3T3 cell transformation protocols, problems and improvements. In Kakunaga T, Yamasaki H (eds) Transformation assay of established cell lines mechanisms and application. IARC Scientific Publications 67, Lyon, pp 165-181... 

Figure 26.2 Transformation protocol algorithm. The steps required to generate trans-formed/immortalized cells and ensure that the cells have the appropriate phenotypic characteristics for the studies intended.

The transformation protocol is the same as described earher in the chimera production section. In this case, I-IO pi of the PCR mixture is used to transform the cells and, after transformation, the mixture is plated out on antibiotic plates. 

It is easy to realise that the first part of the [2,2]-paracyclophane transformation protocol introduces an arbitrary auxiliary functional group. Instead of a methoxy group, the introduction of a phosphane [95], a phosphane oxide [11], an aryl or an alkyl group [10] can be realised. 

The spectra are phase sensitive use the transformation protocol recommended by the instrument manufacturer. Do not use magnitude calculation. 

For hazard classification purposes the results of the dissolution/transformation protocol are compared with existing ecotoxicity data for metals and metal compounds. However, for purposes such as data validation, there might be cases where it may be appropriate to use the aqueous medium from a completed transformation test directly in an OECD 202 and 203 daphnia and fish ecotoxicity test. If the CaCl2.2H20 and MgS04.7H20 concentrations of the transformation medium are reduced to one-fifth of the ISO 6341 medium, the completed transformation medium can also be used (upon the addition of micronutrients) in an OECD 201 algae ecotoxicity test. 

Solutions for High-Efficiency Yeast Transformation Protocol... 

Follow high-efficiency transformation protocol described in Subheadings 3.2.3 and 3.2.4 to create the yeast library. 

HAN, K.H., MEILAN, R., MA, C., STRAUSS, S.H., An Agrobacterium tumefaciens transformation protocol effective on a variety of cottonwood hybrids (genus Populus), Plant Cell Rep., 2000,19, 315-320. 

Recent achievements in molecular biology have created multiple methods that can be utilized to construct mutants by allelic replacements in different bacteria. A variety of temperature-sensitive plasmids, antibiotics resistance markers, and transformation protocols are now available. 

Growth requirements - Genomic/metabolic information -Transformation protocols... 

Dispense the electrocompetent cells in 50-100 p,l aliquots in 1.5 ml polypropylene tubes and proceed as quickly as possible to the transformation protocol (see Protocol 15). 

Presently, no metabolic engineering effort of PTOX biosynthesis has been reported. Rajesh et al. [24] developed an efficient transformation protocol into P. hexandrum embryogenic cells, providing a basis for future gene transfer into PTOX-producing plants to optimize the production systems. 

Much effort is being put into developing a reliable transformation protocol for the oil palm. We are employing both direct DNA uptake and biolistic bombardment to achieve this. Transformation of various tissues, including pollens, young leaves and embryogenic calli, has been attempted. 

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