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Marker antibiotic resistance

The selection of transformed chloroplasts usually involves the use of an antibiotic resistance marker. Spectinomycin is used most routinely because of the high specificity it displays as a prokaryotic translational inhibitor as well as the relatively low side effects it exerts on plants. The bacterial aminoglycoside 3 -adenyltransferase gene (ciadA) confers resistance to both streptomycin and spectinomycin. The aadA protein catalyzes the covalent transfer of an adenosine monophosphate (AMP) residue from adenosine triphosphate (ATP) to spectinomycin, thereby converting the antibiotic into an inactive form that no longer inhibits protein synthesis for prokaryotic 70S ribosomes that are present in the chloroplast. [Pg.62]

Khan, M.S. et al. (1999). Fluorescent antibiotic resistance marker for tracking plastid transformation in higher plants. Nat. Biotechnol. 17 910-915. [Pg.75]

Gay, P. 2001. The biosafety of antibiotic resistance markers in plant transformation and the dissemination of genes through horizontal gene flow. In Safety of Genetically Engineered Crops, R. Custers, Ed. VIB Flanders Interuniversity Institute for Biotechnology, Zwijnaarde, Belgium, pp. 135-159. [Pg.48]

Antibiotic resistance markers. These are genes that code for proteins that confer resistance to specific antibiotics. These markers therefore allow the selection of hosts carrying them. [Pg.418]

Another concern is that the antibiotic-resistance marker genes used in current transgenic plants may make the antibiotics involved useless in treating human diseases.336 It is possible to produce transgenic crops without antibiotic marker genes. Because transgenic crops contain foreign... [Pg.347]

Hygromycin B [29] is an alternative antibiotic resistance marker for the selection... [Pg.665]

Production Strains Devoid of Antibiotic Resistance Markers 238... [Pg.73]

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See also in sourсe #XX -- [ Pg.252 , Pg.254 ]



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Production Strains Devoid of Antibiotic Resistance Markers

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