Transfer spleen cells

Playfair, J. H. L., Papermaster, B. W., Cole, L. J. Focal antibody production by transferred spleen cells in irradiated mice. Science 149, 998-IOOO (1965)-... 

The cell fusion mixture is transferred to a culture medium containing hypoxanthine, aminopterin and thymidine (HAT medium). Unflised myeloma cells are unable to grow as they lack HGPRT. Unflised normal spleen cells can grow but their proliferahon is limited and they eventually die out. The hybridoma cell can proliferate in the HAT medium as the normal spleen cell supplies the enzyme which enables the hybridoma to utilize extracellular hypoxanthine. 

FIGURE 7.7 (See color insert) Adoptively transferred D011.10 transgenicT cells can be identified by expression of CD4+ and KJ-126 in spleen cell suspension from Balb/c mice after ovalbumin (OVA) immunization. Balb/c mice were injected iv with D011.10 spleen cells containing 3-5 x 1 06CD4+KJ-126+ cells and immunized by intraperitoneal injection of 2 mg OVA emulsified in complete Freund s adjuvant 2 days later. OVA immunization increases the frequency of KJ+T cells and alters the expression of various surface molecules consistent with T cell (Tc) activation. 

FIGURE 7.7 Adoptively transferred DO11.10 transgenic T cells can be identified by expression of CD4+ and KJ-126 in spleen cell suspension from Balb/c mice after ovalbumin (OVA) immunization. For description, see page 112. 

Asano, K., Ito, A., Ikeda, K. and Okamoto, K. (1 994) Adoptive transfer of protection to Taenia taeniaeformis infection in rats with immune spleen cells. International Journal for Parasitology 24, 413M15. 

Animal spleens that present the highest antibody levels are collected aseptically, disrupted in the culture medium, and then the spleen cell suspensions are transferred to the centrifuge tube containing the myelomas. The mixture contains 2 X 107 myeloma cells for each 10s spleen cells. These cells are allowed to sediment and are then washed twice with a serum medium and centrifuged. 

Spleen and tumor cells are mixed together with some fusing to form hybridomas. All the cells are transferred to a medium in which the hybridoma cells can grow while the others die. The surviving hybridomas have the spleen cell s ability to produce antibodies and the tumor cell s ability to reproduce. 

Pantich, H. S., Adoptive transfer of EAE with activated spleen cells comparison of in vitro activation by concanavilin A and myelin basic protein, Cell. Immunol., 56, 163, 1980. 

Importantly, Braun and coworkers [15] showed that spleen cells from the tolerant mice, when transferred to normal recipient mice, inhibited the SEB-induced IL-2 and IFN-y response in the recipients. This result formally demonstrated that the SEB-specific tolerance in mice exposed repeatedly to SEB is dominant and mediated by suppression. This inhibition could be reversed by in vivo treatment of recipient mice with neutralizing anti-IL-10 antibodies, suggesting that IL-10-secreting cells in the transferred population mediated the suppression. The... 

Levy EM, Bennett M, Kumar V, et al. 1980. Adoptive transfer of spleen cells from mice treated with radioactive strontium Suppressor cells, natural killer cells, and "hybrid resistance" in recipient mice. J Immunol 124(2) 611-618. 

Tyan ML, McDevitt HO, Herzenberg LA (1969) Genetic control of the antibody response to a synthetic polypeptide. Transfer of response with spleen cells or lymphoid precursors. Transplant Proc 1 548-560... 

Briles and Krause carried out adoptive transfers using 10 spleen cells from SWR/J mice that had produced antibody of restricted heterogeneity to group A streptococcal carbohydrate (118). Recipient mice produced antibodies that were identical to those of the donor by the criteria of electrophoretic mobility and idiotype. 

Figure 5.8 Adoptive immunization experiment production of SE agglutinins after transfer of pre-immunized spleen cells of High or Low responder into normal recipients of the opposite line. Donor immunization 5 x 10 SE intravenously 14 and 4 days before transfer. Transfer one spleen equivalent per recipient intraperitoneally. From Biozzi et al (1971). In Progress in Immunology, p. 529, by courtesy of Academic Press, New York.

Figure 5.9 Cell-transfer experiment transfer of spleen cells from normal High or Low responders into outbred immuno-suppressed recipients. 3 x 10 SE are injected intravenously together with spleen cells. A X rays 950 rad 24 hours before transfer B Cyclophosphamide treatment 6 mg per mouse intraperitoneally 6 hours before transfer.

Mice of High and Low lines were irradiated in order to suppress their immune responsiveness. This suppression is only due to the lymphoc)rtes destruction since it is reversed by the transfer of pure populations of small lymphocytes . Irradiated mice of each line received i.v. 4 x 10 spleen cells from (High x Low)Fj hybrids together with an immunizing dose of 3 x 10 SE. 

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