Tissue lung slices

Ressmeyer AR, Larsson AK, Vollmer E, Dahlen SE, Uhlig S, Martin C (2006) Characterisation of guinea pig precision-cut lung slices comparison with human tissues. Eur Respir J 28 603-611. 

At this time the evidence that hyperoxic-induced lung injury was due to excessive oxygen free radical production was still largely indirect. The next major event which specifically addressed this question was the important experiments of Crapo and colleagues in the early 1980 s [10]. These studies conducted with lung tissue were similar to those performed earlier in liver [11] and heart [12]. Crapo and co-workers found that superoxide production, measured as CN-insensitive respiration, was increased in lung slices when exposed to 100% O2 rather than air [10]. This work was extended to the measurement of O2 production in submitochondrial particles [13]. Clearly then exposure of tissues to elevated concentrations of O2 leads to ROS production, the extent of which, in association with local antioxidant defences, will determine the redox balance of the tissue. 

Salamanders are 550 extant species of amphibians within the family of Caudata [39] (see Figure 2.7). The shape of their bodies is like a lizard, with slender bodies, short noses, and long tails. Salamanders live in or near water, or in wetland. Some salamanders are fully aquatic, some take to the water intermittently, and some are entirely terrestrial. Salamanders are often referred to as having a perfect regeneration capability, because they can heal any structure or cell type of their body entirely [40]. They are able to perform scar-free repair of deep tissue wounds after injury. Salamanders are capable of regenerating lost limbs, damaged lungs, sliced... 

Human, rat Lung slices Demethylation of DMN compared in respiratory tissues from rodents and man 100... 

Nave R, Fisher R, Zech K (2006) In vitro metabolism of ciclesonide in human lung and liver precision-cut tissue slices. Biopharm Drug Dispos 27 197-207. 

Methylxanthines inhibit phosphodiesterase in many tissues, and theophylline is about six times as potent as caffeine [6]. The potentiation of drug effects by methylxanthines has often been interpreted as accumulation of cyclic AMP, but methylxanthines also promote the accumulation of cyclic GMP [37]. Methylxanthines also inhibit adenylate cyclase activity in some tissues, as, for example, the noradrenaline-stimulated adenylate cyclase activity in rat erythrocyte ghosts [38], the vasopressin-stimulated adenylate cyclase activity in toad bladder epithelium [39], the increase in cyclic AMP produced in brain slices by depolarising stimuli and by adenosine [40], and the adenylate cyclase activity in guinea-pig lung particles [41]. Both basal and glucagon-stimulated phosphorylase activity in rat liver slices are inhibited by theophylline [42]. However, methylxanthines also have pharmacological effects which are not related to inhibition of phosphodiesterase [43]. 

S2, S8). Others have reported that adenosine inhibits adenylate cyclase from brain, adipocytes, and lung tissue (FI, M6, Wl). Brain tissues of most animals have very little adenosine deaminase. In other tissues the role of breakdown products must be further explored. The fact that cyclase in intact slices is stimulated while that of broken-cell preparations is inhibited may be related to the so-called latent adenosine deaminase that is mitochondria-bound and inactive in assays of adenosine deaminase in homogenates (M16). 

Postprocessing of all computed tomography (CT) source data sets already begins with the reconstruction of axial slices from the raw data. Careful parameter selection for this step is important because it affects the diagnostic quality of the resulting images. For example, both lung and soft-tissue reconstruc-... 

At autopsy, the heart, lung, liver, kidney and spleen were weighed, and thin slices of these organs, together with tissue from the rectus abdominis, pancreas, salivary gland and duodenum were fixed in absolute alcohol or 10% formalin buffered to pH 6.8. 

Blended polyesters PHB/PHB-co-PHHx have shown strong proliferation of chondroc3des released from the cartilage slices of white rahhits from New Zealand. Also, highly hydrophobic PHB-co-PHV-co-PHHx films were found to significantly increase the adhesion of human hone marrow mesenchymal stem cells (MSCs) compared to PLA and tissue culture plates (TCPs). On the contrary, the hydrophobicity of the PHB-co-PHHx films was found to he responsible for the lack of human embryo lung fibroblast (HELP) cell attachment and proliferation. ... 

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