Tissue analysis imaging

Lemaire R,Tabet J, Ducoroy P, et al. Solid ionic matrixes for direct tissue analysis and MALDI imaging. Anal. Chem. 2006 78 809-819. 

MALD1 Imaging Mass Spectrometry for Direct Tissue Analysis of Pharmaceuticals... 

MALDI IMAGING MASS SPECTROMETRY FOR DIRECT TISSUE ANALYSIS... 

Hahn LJ, Kloiber R, Vimy MJ, Takahashi Y, Lorschei-der EL. Dental silver tooth fillings A source of mercury exposure revealed by whole-body image scan and tissue analysis. FASEB J 1989 3 2641-6. 

In addition, the methodology was applied to fluorescence imaging based on the autofluorescence signals of native molecules in cells or tissues. The imaging system is capable of the rapid acquisition of fluorescence intensities as a function of Em, t, and xy-positions, which is achieved by line illumination of the excitation laser beam. We applied this system to the analysis of a plant defense response, accumulation of phytoalexin in oat leaves, induced by elicitor treatment. In oat leaves treated with an elicitor, we successfully observed weakly fluorescent components, one of which possibly originated from avenanthramide A as a phytoalexin, in addition to the strong fluorescence from chlorophyll molecules. 

Walch, A. Rauser, S. Deininger, S.O. Hofler, H. MALDI imaging mass spectrometry for direct tissue analysis A new frontier for molecular histology. Histochem. Cell Biol. 2008,130,421-134. 

Typically, MALDl has been performed under low pressure conditions (ca 10 Torr). In recent years, intermediate pressure (IP, 10 to 1 Torr) [10,12-14] and atmospheric pressure (AP) MALDl [15,16] systems have been developed to analyze more labile molecules and for tissue analysis [10]. Atmospheric pressure matrix-assisted laser desorption/ionization (AP-MALDl) offers the ability to couple a MALDl source to most commercial instruments, including quadrupole ion traps, because the source uses existing ion optics and vacuum transfer lines of commercial instruments to introduce the ions generated by MALDL A brief discussion of IP-MALDI with ion trap technology is presented, but the primary focus is on the instrumentation aspect of ion trap technology, in particular linear ion trap technology, for direct tissue analysis and imaging MS studies. 

Chughtai, K., Heeren, R.M.A. (2010) Mass spectrometric imaging for biomedical tissue analysis. Chem. Rev., 110, 3237-3277. 

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