Molecular histology

The evaluation of miRNA ISH results requires considerations on the specificity of the signal. Specificity control analysis is an inherent part of molecular histology. For miRNA ISH analyses, there are several ways to address this question (40). Specific for double fluorescence analyses, one key point to address is preventing cross-reaction between the two parallel assays (see Note 7). 

Forslind, B. et al., Aspects on the physiology of human skin. Studies using particle probe analysis. (Invited and accepted paper to special issue of MRT on the molecular histology of the skin), Microsc. Res. Techniq., 38, 373, 1998. 

Brimijoin, S., Rakonczay, Z. (1986). Immunocytology and molecular histology of cholinesterases current results and prospects. Int. Rev. Neurobiol. 28 353 10. 

Edelman, G.M. and Crossin, K.L. (1991) Cell adhesion molecules implications for a molecular histology. Annu. Rev. Biochem. 60 155-190. 

Walch, A. Rauser, S. Deininger, S.O. Hofler, H. MALDI imaging mass spectrometry for direct tissue analysis A new frontier for molecular histology. Histochem. Cell Biol. 2008,130,421-134. 

Key words MALDI imaging, molecular histology, tumor, principal component analysis,... 

As classical histological images can be directly overlaid with the molecular dimension derived from the same tissue section, this type of experiment can truly be called molecular histology. 

Kang et al. [23] compared the clinical, histologic, and molecular responses of normal human skin to topical retinol with that of retinoic acid. Application of retinol and retinoic acid produced epidermal thickening. However, retinol produced less erythema compared with retinoic acid. The authors suggest that these data are compatible with the idea that retinol may he a pro-hormone of retinoic acid. 

The nervous system contains an unusually diverse set of intermediate filaments (Table 8-2) with distinctive cellular distributions and developmental expression [21, 22]. Despite their molecular heterogeneity, all intermediate filaments appear as solid, rope-like fibers 8-12 nm in diameter. Neuronal intermediate filaments (NFs) can be hundreds of micrometers long and have characteristic sidearm projections, while filaments in glia or other nonneuronal cells are shorter and lack sidearms (Fig. 8-2). The existence of NFs was established long before much was known about their biochemistry or properties. As stable cytoskeletal structures, NFs were noted in early electron micrographs, and many traditional histological procedures that visualize neurons are based on a specific interaction of metal stains with NFs. 

Kabbur, M.B., et al., Effect of JP-8 jet fuel on molecular and histological parameters related to acute skin irritation, Toxicol. Appl. Pharmacol., 175, 83, 2001. 

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