Mass spectrometric imaging

Stoeckli M, Staab D, Schweitzer A. Compound and metabolite distribution measured by MALDI mass spectrometric imaging in whole-body tissue sections. Int. J. Mass Spectrom. 2006 260 195-202. 

Site has good software subdivision devoted to matrix-assisted laser desorption/ ionization mass spectrometric imaging (MALDI-MSI, also termed imaging MS or MS imaging). 

Rohner, T. C. Staab, D. Stoeckli, M. MALDl mass spectrometric imaging of biological tissue sections. Mech. Ageing Dev. 2005, 126, 177-185. 

Garden, R. W., and Sweedler, J. V. (2000). Heterogeneity within MALDI samples as revealed by mass spectrometric imaging. Anal. Chem. 72 30-36. 

The sample preparation plays a very important role for the analysis of a drug and its metabolites in biological tissue sections using mass spectrometric imaging. Several factors in IMS sample preparation must be considered, from sample collection to surface treatment prior to analysis in order to produce high quality, reliable, and reproducible results. 

Dill A, Ifa D, Manicke N, Zheng O, Cooks R (2009) Mass spectrometric imaging of lipids using desorption electrospray ionization. J Chromatogr B 877 2883-2889. doi 10.1016/j. jchromb.2008.12.058... 

Sjovall P, Lausmaa J, Johansson B (2004) Mass spectrometric imaging of lipids in brain tissue. Anal Chem 76 4271 1278. doi 10.1021/ac049389p... 

Hankin J, Barkley R, Murphy R (2007) Sublimation as a method of matrix application for mass spectrometric imaging. J Am Soc Mass Spectrom 18 1646-1652. doi 10.1016/j. lasms.2007.06.010... 

Rohner T, Staab D, Stoeddi M (2005) MALDI mass spectrometric imaging of biological tissue sections. Mech Ageing Dev 126 177-185. doi 10.1016/j.mad.2004.09.032... 

Figure 2. MALDI mass spectrometric imaging. A laser is rastered over a tissue sample while acquiring a complete mass spectmm from each position, allowing to generate molecular images for multiple analytes.

Hopfgartner G, Varesio E, Stoeckli M. Matrix-assisted laser desorption/ionization mass spectrometric imaging of complete rat sections using a triple quadrupole linear ion trap. Rapid Commtm Mass Spectrom 2009 23(6) 733—736. 

Distribution Studies of Drugs and Metabolites in Tissue by Mass Spectrometric Imaging... 

In order to use a mass spectrometer as an imaging instrument, it is necessary for the mass spectrometer to be equipped with a rastering function, which allows the sample (or the laser beam) to be rastered in a precise manner to move the laser beam across the tissue surface. A sophisticated data acquisition system is necessary to handle the large amount of data acquired during analysis, and visualization software is essential to create images from the ion intensities of selected ions. Several commercial mass spectrometric imaging instruments are now available with these features. Almost all manufacturers have developed in-house software and have included methods for instrument and image reconstruction, but there are some noncommercial products as well. 

Landgraf RR. Analysis of lipids in nerve tissue by MALDI tandem mass spectrometric imaging. PhD dissertation. University of Florida, Gainesville, FL, 2009, p. 135. 

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