Thin Resin Sections

The surface of acrylic sections is rougher than that of epoxy sections. Moreover, acrylic sections are less crosslinked and more hydrophilic than epoxy sections. As a result, immunostaining reagents penetrate acrylic sections easily, facilitating antigen detection. Exposure of acrylic sections to oxidizing agents worsen both the known instability of these sections under the electron beam and the structural details. 

The staining is carried out with 100 pi of 4% uranyl acetate in 50% ethanol for 1 min in a microwave oven set at a power level of 125,6W, followed by rinsing with 500 ml of distilled water. This is followed by staining for 1 min with 100 pi of triple lead citrate (Sato et al., 1988) and then rinsing with 500 ml of distilled water. This lead citrate staining solution avoids the production of artifactual lead carbonate precipitates. 

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ANTIGEN RETRIEVAL ON THIN RESIN SECTIONS USING AUTOCLAVING... 

In addition to antigen retrieval on sections of paraffin-embedded tissues for light microscopy, antigen retrieval can be carried out on thin resin sections for electron... 

RAPID STAINING OF THIN RESIN SECTIONS IN MICROWAVE OVEN... 

Stain resin sections with 1% methanolic uranyl acetate and lead citrate according to routine electron microscopy procedures. If ultra-thin frozen sections are being used, stain with 2% aqueous uranyl acetate, neutral uranyl acetate, and embed in methyl cellulose before examination (7). 

Such blends are attractive for a variety of end-use articles in the automotive market, especially for under hood and various exterior components. Some applications, for example connectors, have very thin wall sections and therefore require resins that have very low viscosities in order to completely fill the molding tools. The melt flow can be enhanced by mixing with a dendritic polyester resin. The inclusion of only 0.5% of a dendritic polyester resin can increase the melt flow rate by as much as 100%. °... 

When thick-walled hollow molded artieles and molded articles with a shape having thick-walled sections and thin-walled sections are produced, the molded article is produced in two parts. These parts are eventually eon-nected by welding. Alternatively, the two parts are placed in another mold so that they face each other at the parting lines. Then, molten resin is injected about the periphery of the opposed faces to connect the parts. This technique is addressed as insert molding, or overlap molding. Other welding methods include ... 

For thin section electron microscopy cells were frozen in LN2-cooled Freon 22 then substituted at -80°C in 2% glutaraldehyde in anhydrous acetone for 3 days then in 1% OSO4 in acetone for 2 days. Cells were then brought up to room temperature and embedded in Spurr s resin. Sections were stained with uranyl acetate and lead citrate. 

A liquid-crystalline polyester resin composition is designed by incorporating a liquid-crystalline polyester obtained by polymerizing monomers of an aromatic hydroxycarboxylic acid in the presence of an imidazole compound. The composition also includes a mica (50-100 phr) having a volume average particle diameter of 40 pm or less and a specific surface area of 6 m /g or less. The liquid-crystalline polyester resin composition has melt fluidity sufficient to be molded into a connector having an ultra-thin wall section. The coimector, thus molded, has sufficiently suppressed warp and sufficient soldering resistance [171]. 

Different pyrolytic techniques may be used, the pyrosylates being examined by infrared, Raman spectroscopy or other techniques. Microscope techniques have improved over the last few years and this now means that very smal 1 regions of a sample may be successfully examined. The use of infrared microscopes has proved to be invaluable for the examination of laminates. It is often possible to microtome a thin cross-section from a laminate (for example, methylmethacrylate resin is sometimes used for this purpose) and then to examine individual layers in transmission using an infrared or Raman microscope system. 

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