Sectioning, electron microscopy

Marsh et al. (1983) have shown that SFV can still infect BHK-21 cells. Moreover, the release of nucleocapsid into the cytoplasm (as measured by the ribonuclease assay) proceeds, albeit 40% more slowly than at 37 C. Thin-section electron microscopy confirms that SFV particles are not transferred to lysosomes at this temperature. 

Fig. 23 (a) Small-angle X-ray diffraction profile of EDOPC lipoplexes at 4 1 lipid/DNA weight ratio (arrow points to the peak originating from DNA-DNA in-plane correlation) inset, thin-section electron microscopy image of EDOPC lipoplexes (reproduced with permission from [81] copyright (2007) Elsevier), (b) Electron density profiles of the lipid bilayer in presence and in absence of DNA [16] (copyright (2000) Biophysical Society)... 

Detection of viral particles by thin section electron microscopy... 

Smiley JF, Goldman-Rakic PS (1993) Heterogeneous targets of dopamine synapses in monkey prefrontal cortex demonstrated by serial section electron microscopy A laminar analysis using the silver-enhanced diaminobenzidine sulfide (SEDS) immunolabelling technique. Cereb Cortex 3 223-238. 

The biopsy material should be observed under UV fluorescence (366 nm so-called Wood light) to rule out porphyria, (s. fig. 7.10) Only after UY-fluorescence assessment has been carried out can the biopsy material be fixed in the formalin solution. A short written description of the biopsy material should then be made. Depending on the individual diagnostic issue in question, the material is prepared in compliance with special regulations (frozen section, electron microscopy, RNA detection, etc). 

Most STEM projects have mass measurement as a major focus. Most of cryo-EM, negative stain EM, and embedding/sectioning electron microscopy... 

Byers, B., and Goetsch, L. (1991). Preparation of yeast cells for thin-section electron microscopy. In Methods in Enzymology (C. Guthrie and G. R. Fink, eds.), Vol. 194, pp. 110-131. Academic Press, San Diego, CA. 

Reassembly of nuclei can be followed most easily by phase-contrast microscopy. During the course of the assembly reaction clusters of chromosomes will be observed to fuse into single masses of chromatin, which will eventually become spherical in shape as decondensation progresses. At this point, thin-section electron microscopy should reveal the presence of a continuous double membrane studded with nuclear pore complexes surrounding each chromatin mass. Assembly of individual nuclear envelope components such as nuclear lamins or nuclear pore complex proteins can best be analyzed by immunofluorescence microscopy (Burke and Gerace, 1986). This is performed as follows. 

For thin section electron microscopy cells were frozen in LN2-cooled Freon 22 then substituted at -80°C in 2% glutaraldehyde in anhydrous acetone for 3 days then in 1% OSO4 in acetone for 2 days. Cells were then brought up to room temperature and embedded in Spurr s resin. Sections were stained with uranyl acetate and lead citrate. 

The characterisation of the as-welded microstructure in different regions of the 9Cr-lMo weldment has been carried out by extensive cross section electron microscopy. The observed microstructures at varying distances from the heat source have been correlated with the temperature isotherms predicted for each region. The repartitioning of solutes, namely Cr and Mo between the weld metal and heat affected zone has been established. 

A novel method for the study of diffusion through catalytic layers has been recently proposed by Novak et al. [20]. The authors predicted the effective dififusivity by employing a detailed pore scale model. This model is based on the digital reconstruction of the porous layers as 3D matrices using the information from cross-section electron microscopy and particle size analysis. 

There are several methods by which the crystallite-thickness distribution can be determined. These include thin-section electron microscopy, analysis of the Raman longitudinal acoustic mode, and measurement of the small-angle X-ray-scattering long period. These methods give concordant results for narrow crystallite-thickness distributions. However, agreement is not usually observed when the thickness distribution is broad. However, when cognizance of this dispersity is taken, a rational... 

Morphologies were characterized by thin-section electron microscopy. Samples were first stained with osmium tetroxide (which reacts with residual double bonds) they were then embedded in an epoxy matrix, trimmed to a truncated pyramid shape, and microtomed using a Porter MT-2 ultra-microtome equipped with a diamond knife. Transmission electron micrographs were taken using a Phillips 300 electron microscope. 

The ultrastructural features of chloroplasts in leaves of Columbia wild-type Arabidopsis thaliana (L) Heyn and mutant strains JB67 and LK3 isolated therefrom have been examined by thin section electron microscopy. Leaves from 3 week old plants grown at 22°C under standard conditions were processed in tendem. Tissue was fixed in 4% gluterdi-aldehyde, sectioned and stained with uranyl acetate and lead citrate. The sections were examined by transmission electron microscopy and morphometric features of thin sections through chloroplasts were analysed (see Table 1). 

Thin-section electron microscopy of polytene chromosomes Protocol 39... 

Hamzei-Sichani F, Kamasawa N, Janssen WG et al (2007) Gap junctions on hippocampal mossy fiber axons demonstrated by thin-section electron microscopy and fireeze firac-tme replica immunogold labeling. Proc Natl Acad Sci U S A 104 12548-12553... 

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