Densitometers scanning

Fig. 2. Tic densitometer scans showing the resolution of isoproterenol on a hpflc siUca-gel plate obtained using a mobile phase consisting of 6.8 mM (1 R)-(—)-ammonium-10-camphorsu1fonic acid in 75 25 (v/v) methylene chioride methano1. (a) 254 nm, (b) 275 m, (c) 300 nm.

In the following, an example from Chapter 4 will be used to demonstrate the concept of statistical ruggedness, by applying the chosen fitting model to data purposely corrupted by the Monte Carlo technique. The data are normalized TLC peak heights from densitometer scans. (See Section 4.2) ... 

Densitometer scans of developed gel electrophoresis of DNA following oxidative damage by the Fenton reagent. 

Summary of Densitometer Scanning Results of DNA Damage When Subjected to the Fenton Reaction in the Presence of Various Antioxidants (at a concentration of 50 pM each)... 

Improvements in the collection and processing of observed intensities are now being developed and applied. We should expect in the future to request the two-dimensional densitometer scan from authors if one wishes to re-examine a particular structure determination. 

Fig. 3. Densitometer scans showing electrophoretic separation of Chl-protein complexes in P. laminosum. Samples were pretreated with LDAO at a ratio LDAO Chl=3.5 l and treated with SDS at a ratio SDS Chl=20 l as described under Materials and Methods, (a) membrane fragments isolated according to Stewart and Bendall (1980), (b) PSI-enriched particles. The area of the peaks corresponding to the free pigment (FP) bands was similar in both cases.

Results and Discussion. X—ray diffraction densitometer scans are presented In Figures 2 for the 18 EG and Figure 3 for the 30 EG series for RIM polyurethanes. DSC and Storage Modulus traces (El) for the same specimens are shown In Figures 4, 5 and 6, and flexural modulus, elongation, Impact strength, and heat sag data are given In Table I. 

Figure 8.26 P P]ADP-ribosylation of platelet membrane proteins after pretreatment with iloprost. Platelets were incubated for 24 h in the absence (lanes 1—3) or presence (lanes 4—6) of 10 )Umol iloprost. Platelet membranes were prepared, and ADP-ribosylation of membrane proteins performed in the presence of cholera toxin (A subunit). The gels were loaded with (lanes 1-6) 3.95, 9.88, 39.5, 3.05, 7.63, 30.5 fig protein. The figure shows the autoradiograph of the 6 lanes, and a densitometer scan of lanes 3 and 6. In a control experiment, there was no significant labelling of the platelet membrane proteins in an incubation from which cholera toxin had been omitted. (Reproduced from reference 35 with permission)...

Fig. 4. Manual sequencing of plasmids with topoisomerase V. Autoradiogram of the gel (A) and densitometer scans (B) of the lanes (+Topo V, dotted lines, —Topo V, solid lines), fmol DNA sequencing kit from Promega was used.

Distance (in mm) from the base of an esterase peak to its apex on densitometer scan,... 

Small Angle X-Ray Scattering Studies. For polyurethanes, a Kratky camera was used while for polyurethaneureas, SAKS patterns were initially recorded in photographic films and a densitometer was used to scan the intensity as a function of angle. In order to verify that the two techniques provide similar results, one of the polyurethanes (2,4 TDI-BD-PTMO 1000 (2 1 1)) was studied by the photographic method. The densitometer scan of SAKS profile was exactly the same as observed by a Kratky camera. 

Fig. 1 Immunoblots of acid extracts of nuclear preparations from mouse epidermal cells JB6 (clone 41) which had been treated with active oxygen produced by xanthine/xanthine oxidase (50 pg/ml xanthine plus 5 Xg/ml xanthine oxidase) for 30 min or 5 pg/ml MNNG for 20 min. The extracts were purified on a boronate affinity column and the proteins separated on 15% polyacrylamide gels (34). A polyclonal rabbit antibody against histone H3 (37) was used for the immunoblot and the blot was reacted with [i Sjj-iabeled donkey anti-rabbit IgG. Densitometer scannings are shown at the right side of the autoradiogram and were obtained with a Zeineh "soft laser" scanning densitometer.

Fig. 4. A The level of calmodulin mRNA in Merit corn root tips that were left in dark (D) or exposed to light (L). Seedlings (48-h-old) were kept either in dark or exposed to light for 40 min. Then the root tips (2 mm) were excised, frozen immediately in liquid K, and used for poly(A) isolation. Two fig of RNA was probed with radiolabeled pPCM-1. The autoradiogram and densitometer scanning results are presented on the lower and upper part of the figure, respectively. B Light-regulated positive gravitropism in Merit corn roots. Corn seedlings (48-h-old) were kept dark (D) or exposed to light for 10 min and left in the dark (L) for 6 h [20]...

Errors in sample application are among the most significant sources of inaccuracy. The technique of spotting can have a marked effect on delivery volumes between I and 5 pL. A constant slat ting zone size should be maintained so that a given amount of substance applied in the form of different volumes will yield spots with identical areas, whereas different amounts spotted at constant volume will exhibit a linear relationship in a plot of peak area (from recordings of densitometer scans) versus mass. 

Documentation can also be performed with a densitometer, scanning the whole gel and obtaining information of the intensity as a function of location. The densitometer can also be used for quantitative determinations. 

Figure 2.1 Chromatographic resolution determined from spots or densitometer scan as the ratio of the separation of zone centers to the average zone width. X = origin and F = solvent front of the TLC plate the arrow shows the direction of solvent development. The peaks represent the scan of the separated zones.

Figure 22.1 Densitometer scans of (A) aspirin (34.8 pg), (B) caffeine (30.1 pg), and (C) phenacetin (0.50 pg) at 254 nm on phosphor-containing silica gel HPTLC plates. The attenuation setting of the integrator/recorder was x6 in each case.

CHROMATOGRAM. The series of zones on or in the layer after development (the developed TLC plate), or the record of the separation presented as a densitometer scan, photograph, photocopy, or video image. 

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