Thin layer chromatography phytosterols

Lebovics, V.K. 2002. Determination of cholesterol oxidation products by thin-layer chromatography. In Cholesterol and Phytosterol Oxidation Products Analysis, Occurrence, and Biological Effects (F. Guardiola, P.C. Dutta, R. Codony, G.P. Savage, eds.), pp. 101-113, AOCS Press, Champaign, IL. 

The fatty/waxy products contained the lipophilic substances, including fatty oils, waxes, resins and colorants. Valuable pharmacological effects were proved for some minor constituents of these products (e.g. triterpenes, diterpenes, sterols and carotenoids). Thin layer chromatography and on-line UV-VIS spectroscopy were used for the quick identification and quantity determination of these compounds using authentic samples as standards. The SFE method proved favorable in terras of both extraction yield and speed of carotenoids. The CO2 extracts of the lavandin, clary sage and thyme have been enriched in triterpenic compounds (a-es P-amyrin, oleanic acid, ursolic acid, etc.) and phytosterols. Both free and esterified triterpenoids were present in the extracts of the different samples. Furthermore camosol and other diterpenes were detected in the SFE extract of Lamiaceae plants. The fatty acid composition was only slightly different for extracts obtained by SFE and conventional hexane extraction. 

Official methods for the analysis of phytosterols in olive oil (European Union Commission, 1991 International Olive Oil Council, 2011) involve several steps. First, olive oil saponification is required for the separation of saponifiable and unsa-ponifiable olive oil fractions with diethyl ether (washing the extract with water), and then separation by thin-layer chromatography (TLC) on silica gel plates and derivatization of the sterols. The sterols as Irimethylsilyl derivatives are quantified, afterwards, by means of a capillary GC analysis with FID (flame ionization detector) [50,75],... 

Naumoska, K. and Vovk, 1. 2015. Analysis of triterpenoids and phytosterols in vegetables by thin-layer chromatography coupled to tandem mass spectrometry,... 

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