Thin-layer chromatography , coupling

He, W. Shanks, R. Amarasinghe, G. Analysis of additives in polymers by thin-layer chromatography coupled with Eourier transform-infrared microscopy. Vibr. Spectrosc. 2002, 30, 147-156. 

Thin-layer chromatography coupled with on-line flame ionization detection (TLC-FID) has been developed for the analysis of organic substances which show no UV absorption and no fluorescence and which present difficulties by GC analysis. 

Busch, K. L. Thin-Layer Chromatography coupled with Mass Spectrometry , in Handbook of Thin-Layer Chromatography, J. Sherma, B. Eried Chromatographic Science Series, Vol. 55, 1991, 183-209, Marcel Dekker, New York... 

Eleven PAH were quantified by means of glass capillary gas chromatography and two-dimensional thin-layer chromatography coupled with in situ fluorescence spectrometry. 

Thin-Layer Chromatography Coupled with Fluorometry. Aliquots of the prepared samples stored in the pear-shaped flasks are spotted on the thin-layer plates. The development of these plates for separation of the individual compounds can be done in one or two directions which takes place in an equilibrated chamber first with a mixture of toluene/n-hexane/n-pentane (5 90 5) to a distance of 15-16 cm. After drying, the plates are developed in the second direction with a mixture of methanol/diethyl ether/water (6 4 1). A typical two dimensional TLC of a PAH standard is shown in Figure 3. 

Busch, K. L. (1996) Thin-layer chromatography coupled with mass spectrometry in Handbook of Thin-layer Chromatography (eds J. Sherma and B. Fried), Marcel Dekker, New York, pp. 241-72. 

Anderson, R.M., Busch, K.L. (1998) Thin-layer chromatography coupled with mass spectrometry interfaces to electrospray ionization. Journal of Planar Chromatography, 11, 336-341. 

Bush, K.L. 1991. Thin layer chromatography coupled with mass spectrometry, in Handbook of Thin Layer Chromatography, Sherma, J. and Fried, B., Eds., CRC Press/ Taylor Francis Group, Boca Raton, EL, Chapter 8. 

Lv, Y, Lin, Z., Tan, T., and Svec, F. Preparation of porous styrenic-based monolithic layers for thin layer chromatography coupled with matrix-assisted laser-desorption/ ionization time of flight mass spectrometric detection, J. Chromatogr. A, 1316,154-159, 2013. 

Chen, Y. and Schwack, W. 2014, Rapid and selective determination of multi-sulfonamides by high-performance thin layer chromatography coupled to fluorescent densitometry and electrospray ionization mass detection, J. Chromatogr. A, 1331 108-116. 

Kuwayama, K., Tsujikawa, K., Miyaguchi, H., Kanamori, T., Iwata, Y.T., and Inoue, H. 2012, Rapid, simple, and highly sensitive analysis of drugs in biological samples using thin-layer chromatography coupled with matrix-assisted laser desorption/ionization mass spectrometry. Ana/. Bioanal. Chem., 402 1257-1267. 

Naumoska, K. and Vovk, 1. 2015. Analysis of triterpenoids and phytosterols in vegetables by thin-layer chromatography coupled to tandem mass spectrometry,... 

Bakry, R., Bonn, G. K., Mair, D., and Svec, F. 2007. Monolithic porous polymer layer for the separation of peptides and proteins using thin-layer chromatography coupled with MALDI-TOF-MS, Anal. Chem., 79 486 93. 

Nakamura, K., Suzuki, Y., Goto-Inoue, N., Yoshida-Noro, C., and Suzuki, A. 2006. Structural characterization of neutral glycosphingoUpids by thin-layer chromatography coupled to matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight MS/MS, Ana/. Chem., 78 5736-5743. 

Sabino, B.D., Sodre, M.L., Alves, E.A., Rozembaum, H.F., Alonso, F.O.M, Correa, D.N., Eberlin, M.N., and Romao, W. 2010. Analysis of street Ecstasy tablets by thin layer chromatography coupled to easy ambient sonic-spray ionization mass spectrometry, Braz. J. Anal. Chem., 1 6-11. 

Most commonly used is the recording of UVA IS spectra, which are compared with spectral libraries. However, more informative is the recording of a FTIR- or Raman-spectrum in situ. These relatively recent developments to measure diffuse reflectance can now be carried out routinely. The in situ employment of MS after TLC detection is described in detail elsewhere (see Chapter 9, Thin Layer Chromatography Coupled with Mass Spectrometry), as well as the employment of radioactively labelled substances (see Chapter 12, Thin-Layer Radiochromatography). 

For this in situ identification method especially FAB (Fast Atom Bombardment), Liquid SIMS (Secondary-Ion MS), or laser desorption are employed as ionization techniques (49,50). The analytes are sputtered from the TLC foil (Fig. 26 (51)), or the TLC plate is placed on a movable table. More details are described in Chapter 9, Thin Layer Chromatography Coupled with Mass Spectrometry. 

Thin-Layer Chromatography Coupled with Mass Spectrometry... 

Colorimetry, capillary electrophoresis (CE), ° mass spectrometry (MS), gas chromatography (GC), high-performance liquid chromatography (HPLC), thin-layer chromatography coupled with different detectors, and spectral techniques are the most commonly employed methods for trace environmental analysis of pesticides and also are part of regulations in monitoring the environmental pollutants. 

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