Thin-layer chromatography of amino acids

A. Niederwieser, in Methods in Enzymology, Vol. XXV, C. Hirs and S. Timasheff, Editors (1972), Academic Press (New York), pp. 60-99. Thin-layer chromatography of amino acids. 

Thin-layer chromatography of amino acid-phenylthiohydantoin derivatives on silica gel plates, (a) Separation is done in a 98 2 mixture of chloroform and ethanol. (b) This is followed by further separation using an 88 2 10 mixture of chloroform, ethanol, and methanol. More sophisticated procedures, using column chromatography, give superior resolution and improved sensitivity. Automated sequencers always use such procedures. A general description of the use of columns is given in chapter 6. 

Thin-layer chromatography of amino acids was carried out on layers of Kieselgel H (Anderman and Co., Ltd., London, S.E. 1), which were developed first in butan-l-ol acetic acid-water and dried in air overnight. 

L. Lepri, P. G. Desideri, andD. Heimler, Reversed-phase and soap-thin-layer chromatography of amino acids, /. Chroma-togr. 195 65 (1980). 

B23. Brenner, M., Niederwieser, A., and Pataki, G., Thin layer chromatography of amino acid derivatives using Kieseigel G. N-(2,4-dinitrophenyl)-amino acid and 3-phenyl-2-thiohydantoin). Experientia 17, 145-153 (1961). 

H16. Horton, D., Tanimura, A., and Wolfrom, M. L., Two-dimensional thin-layer chromatography of amino acids on microcr3retalline cellulose. J. Chromatogr. 23, 309-312 (1966). 

Two-Dimensional Thin-layer Chromatography of Amino Acids on Microcrystalline Cellulose, D. Horton, A. Tanimura, and M. L. Wolfrom,/. Chromatogr., 23, 309-312... 

Brenner, M., Niederwieser, A. Thin Layer Chromatography of Amino Acids in Methods of Enzymology, Vol. 11 (Hirs, C. H. ed.) pp. 39-59, New York Academic Press 1967... 

Lu, T. and Olesik, S.V. 2013. Electrospun polyvinyl alcohol nltra-thin layer chromatography of amino acids, J. Chromatogr. B, 912 98-104. 

It is striking that the jK/-values generally do not exceed 0.7 in onedimensional thin-layer chromatography of amino acids. Only about 2/3 of the distance from start ta front is thus utilised in separation in all solvents investigated by us. Increasing the water content of the solvent raises small J /-values more than larger, so that the effective separation path is diminished. 

Sherma, J., and Touchstone, J. C. (1974). Quantitative thin layer chromatography of amino acids using fluorescamine reagent. Anal. Lett. 7 279-287. 

G. Pataki, Techniques of Thin Layer Chromatography in Amino Acid and Peptide Chemistry,... 

B. Basak, U. K. Bhattacharyya, and S. Laskar, Spray reagent for the detection of amino acids on thin layer chromatography plates, Amino Acids, 4 193(1993). 

P7. Pataki, G., Techniques of Thin-Layer Chromatography in Amino Acid and Peptide Chemistry, Rev. Ed. Ann Arbor Sci. Publ., Ann Arbor, Michigan, 1968. 

V3. Van Sumere, C. F., Cottenie, J., and Teuchy, H., An improved qualitative, monodimensional thin layer chromatography for amino acids, using mixed layers of silica gel and cellulose and a combination of solvent systems. Arch. Int. Physiol. Biochim. 76, 965-967 (1968). 

Incubation of this Na-K ATPase preparation with phosphatidylserine decarboxylase leads to complete conversion of the 31 phosphatidyl serine molecules present per molecule of Na-K ATPase, again without significant reduction in Na-K ATPase activity. In this case the sensitivity of the phosphatidylserine assay (thin layer chromatography and amino acid analysis) is such that it can be stated that less than one phosphatidylserine molecule per molecule of enz3rme is left. 

Hydroxypyrimidines have been detected in the Murchison, Murray, and Orgueil carbonaceous chondrites in abundances similar to those of amino acids [7]. Earlier analyses of the Orgueil meteorite By thin layer chromatography of organic extracts indicated the presence of melamine, ammeline, adenine, and guanine [8]. Although these could not be confirmed by Folsome, et al., [7] using GC/MS, recent studies by Schwartz [11] and by Hayatsu, et al., [12] have shown that these constituents of the nucleic acids may indeed exist in the carbonaceous chondrites. 

Thin-layer chromatography of dansyl-amino acids on a polyamide plate. A After solvents 1 (1.5% formic acid) and 2 (toluene-acetic acid). B After solvents 1,2, and 3 (ethyl acetate-methanol-acetic acid). See text for further details. 

Day 2 Thin Layer Chromatography of the DNP-Amino Acid and Paper Chromatography of the Acid-Hydrolyzed Dipeptide... 

Dll. Deyl, F., and Rosmus, J., Thin-layer chromatography of dansyl amino acid derivatives. J. Chromatogr. 20, 514-520 (1965). 

F15. Frentz, R., Thin-layer chromatography of urinary amino acids. Ann. Biol. Clin. Paris) 23, 1145-1155 (1965). 

J4. Jeppsson, J. O., and Sjoquist, J., Thin-layer chromatography of PTH-amino acids. Anal. Biochem. 18, 264-269 (1967). 

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