Spray reagents for

Note The dipping reagent, which can also be applied as a spray reagent, can be employed on cellulose and silica gel layers. A 3% solution of 2,2 -bipyridine in 40% thioglycolic acid can be employed as a specific spray reagent for the detection of iron (red coloration) [7],... 

Patil VB, Sevaikar MT, Padalikar SV. 1987. Specific spray reagent for the detection of endosulfan by thin-layer chromatography. J Chromatogr 396 441-443. 

It may accept more corrosive spray-reagents, for example cone, sulphuric acid, phosphoric acid, phosphomolybdic acid, perchloric acid on antimony trichloride and the sprayed plates could be heated upto 110-120 °C without any darkening whatsoever,... 

Aqueous or methanolic ferric chloride is a general spray reagent for phenolic compounds and gives a blue-black coloration with flavonoids. Similarly, Fast Blue Salt B forms blue or blue-violet azo dyes. 

Early applications of thin-layer chromatography in corticosteroid analysis were mainly based on use of phenylhydrazine in sulfuric acid as a spray reagent for derivatization of the analytes (529, 530). Later applications involved use of other spray reagents including phosphoric acid in methanol, tetrazolium blue. 

Method (manual). An aliquot portion (0.1 ml) of effluent containing the amino acid is mixed with 3 ml of a solution containing 1.5 ml of o-phthalaldehyde (10 mg/ml in ethanol), 90 ml of sodium tetraborate buffer (0.05 M, pH 9.5) and 1.5 ml of a solution of 2-mercaptoethanol (5 mg/ml) in ethanol. The latter reagent should be freshly prepared each day. The reaction mixture is permitted to stand for 5 min and the intensity of fluorescence is measured in a fluorimeter (340-nm excitation, 455-nm emission). The buffered reagent may be also useful as a spray reagent for amino acids separated by TLC, although such an investigation has not been reported. 

S. Gupta, S.K. Handa and K.K. Sharma, A new spray reagent for the detection of synthetic pyrethoids containing a nitrile group on thin-layer plates, Talanta, 45 (1998) 1111-1114. 

Partridge, S.M., Aniline hydrogen phthalate as a spraying reagent for chromatography of sugars, Nature, 164, 443, 1949. 

Harley-Mason, J. and Archer, A.A.P.G., p-Dimethylamino-cinnamaldehyde as a spray reagent for indole derivatives on paper chromatograms, Biochem. J., 69, 60, 1958 Chem. Abs., 52, 18600g, 1958. 

Sattler, L. and Zerban, F.W., New spray reagents for paper chromatography of reducing sugars, Anal. Chem., 24, 1862, 1952 Chem. Abs., 47, 1543d, 1953. 

B. Basak, U. K. Bhattacharyya, and S. Laskar, Spray reagent for the detection of amino acids on thin layer chromatography plates, Amino Acids, 4 193(1993). 

Manganous chloride-sulphuric acid is a useful spray reagent for t.l.c. of sterols and bile acids, giving characteristic colours. A simple technique for thin-layer... 

Frei, R. W., Miketakova, V. A reflectance spectroscopic study of 4-(2-thiazolyl-azo)resor-cinol (TAR) as a spray reagent for cobalt, copper and nickel on thin-layer chromatograms. Mikrochim. Acta 290 (1971)... 

W4. Waldi, D., Spray reagents for thin-layer chromatography. In Thin-Layer Chromatography. A Laboratory Handbook (E. Stahl, ed.), pp. 483-502. Academic Press, New York, 1965. 

In Inorganic Chemistry, typical spray reagents for cations include potassium iodine (0.2%, aqueous), hydrogen sulphide (saturated aqueous solution), ammonium sulphide (0.2 N, aqueous), quercetin (0.1%, alcoholic), l-(2-pyridylazo)-2-naphthol (PAN) (0.2%, methanolic), oxine (8-hydroxyquinoline) (1% methanolic, view under visible and UV light), and sodium rhodizonate (0.5%, aqueous). Reaction with dithizone to produce coloured dithizonate chelates of many metals is particularly suitable if quantitative spectrometric analysis (in situ or after elution) is to be carried out. Anions are detected with bromocresol purple (0.1%, alcoholic), 1% ammoniacal silver nitrate + 0.1% alcoholic fluorescein/UV light, zirconium alizarin lake (0.1% in HC1 solution), and ammonium molybdate (1%, aqueous) followed by SnCl2 (1%) and HC1 (10%). Typical detection limits range from 10 ng (10 9g) to several pg (10 6g). 

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