Thin film coatings for stem cell technologies

Fernandez, N. Rogers, J.D. Whittle University of South Australia, Adelaide, SA, Australia 

Since the 1960s, surface modification has been used to enable cells to be cultured on normally inhospitable plastic. The marketing of plasma-treated polystyrene microplates, flasks, roller bottles and dishes has allowed cell culture to be carried out quickly and cheaply on disposable base materials, avoiding the need to culture in glass (in vitro) and reducing the possibility of cross-contamination. More recently, the challenges associated with the culture of primary and pluripotent cells and a focus on the reduction or elimination of animal-derived products in cell culture have stimulated the development of novel surface-coated cell culture products. 

In this chapter, the fundamental processes contributing to successful cell culture are reviewed, and a number of different approaches to engineering novel surfaces are described. The major challenges are discussed in the context of ongoing research efforts in this field. 

2 Thin film coatings for culture of normal cells 

Thin Film Coating for Biomat rials and Biomedical Ai catimis. litlp i9dx.doLm l0.1016ffi978-l- re242-4S3- .00009 2 Copyright 2016 Elsevier Ltd. All rights reserved. 

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Thin film coatings for stem cell technologies... 

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