Tetrahydrochloride

Diaminobenzidine tetrahydrochloride (2H2O) [7411-49-6] M 396.1, m >300°(dec), pK ,t(D) --3.3, pKEst(2) 4 7 (free base). Dissolved in water and ppted by adding cone HCl, then dried over solid NaOH. 

Zsila F, Fitos I, Bikadi Z, Simonyi M, Jackson HL, and Lockwood SF. 2004. In vitro plasma protein binding and aqueous aggregation behavior of astaxanthin dilysinate tetrahydrochloride. Bioorganic Medicinal Chemistry Letters 14(21) 5357-5366. 

Gunter and co-workers have applied this methodology to the condensation of norbomene derivative 90 (block A, Scheme 32) with epoxide 94 (block B, Scheme 33) <98S593>. Thus, using the Crossley porphyrin-a-dione 47, condensation with 1,2,4,5-benzenetetramine tetrahydrochloride and then the product with a strained dione, gave 90 as block A. 

Fluorine-containing aromatic poly(benzimidazole)s are synthesized by direct polycondensation of 2,2-bis(4-carboxyphenyl)-l, 1,1,3,3,3-hexafluoropropane (15) with 3,3 -diaminobenzidine tetrahydrochloride (23) (Scheme 13) and 1,2,4,5-benzenetetramine tetrahydrochloride (24) (Scheme 14) in PMMA or PPA.24... 

Chromogen solution 3, 3 diaminobenzidine tetrahydrochloride (DAB) (Sigma St. Louis, MO) 30% hydrogen peroxide (H2O2) see Note 3). 

Primary, secondary and tertiary aliphatic amines are efficiently converted to nitro compounds in 80-90 % yield with dimethyldioxirane, a reagent prepared from the reaction of oxone (2KHSO5-KHSO4-K2SO4) with buffered acetone. Dimethyldioxirane (DMDO) has been used for the synthesis of 1,3,5,7-tetranitroadamantane (71) from the corresponding tetraamine as the tetrahydrochloride salt (70) and is an improvement over the initial synthesis using permanganate anion (Table 1.7). ° Oxone is able to directly convert some aromatic amines into nitro compounds. 

Boyer and co-workers" also reported the synthesis of the guanidine tricycle (122), prepared as the tetrahydrochloride salt from the condensation of two equivalents of guanidine with 1,4-diformyl-2,3,5,6-tetrahydroxypiperazine in concentrated hydrochloric acid. Treatment of the tricycle (122) with absolute nitric acid yields thebis-nitrimine (123), whereas the same reaction with nitric acid-acetic anhydride yields HHTDD (117). 

Ethane-tetracarboxylic Acid Tetrazide, (NsOC)2CH.CH(CON3)2 mw 306.17, OB to C02 —47%, N 54.9%, wh cryst, expl when contacted with a flame. It was prepd from the tetra-hydrazide tetrahydrochloride ia dil cbilled HC1 by addn of aq NaN02, at best in 25% yield and only from no more than 3g of starting material (Ref 5)... 

Actual transfer distance along the primary DNA ds Interduplex center-to-center separation Spermine tetrahydrochloride Dodecyltrimethylammonium bromide Octadecyltrimethylammonium bromide Poly-L-lysine hydrobromide Polyethylenimine hydrochloride Ionization energy... 

Develop the peroxidase in 3,3-diaminobenzidine tetrahydrochloride (DAB) solution 3 ml DAB stock solution and 12 drops hydrogen peroxide (30% w/v) are dissolved in 400 ml TBS incubate the specimen with this solution for 10 min (perhaps longer, control with microscope) Wash with tap water... 

Triethylenetetramine tetrahydrochloride [4961-10-4] M 292.1, m 266-270 . Crystd repeatedly from hot water by pptn with cold EtOH or EtOH/HCl. Washed with acetone and abs EtOH and dried in a vacuum oven at 80°. 

Thus, in analogy to the above outlined procedure cyclen is converted to 12 and without isolation reacted with 4,4-dimethyl-3,5,8-trioxabicyclo [5.1.0] octane to give the formyl derivative 13 which also without isolation is treated with aqueous hydrochoric acid under heat. Under these conditions both the formyl group is removed and the dioxepanyl ring gives rise to the free butrol side chain yielding the monosubstituted cyclen derivative in the form of its tetrahydrochloride 16. The overall yield for this reaction sequence is about 81%... 

The starting linear amine, N, IV -bis(3-aminopropyl)-l, 3-propanediamine, is prepared using the procedure for N,A -bis(2-aminoethyl)-l,3-propanediamine,2 which consists of the condensation of 1,3-dibromopropane with 1,3-diamino-propane. The resulting amine is purified by vacuum distillation from barium oxide, bp 135-136° at 1 torr, 65-70% yield. The tetraamine forms a solid hydrate when exposed to moist air its tetrahydrochloride decomposes at 297°. 

BIPHENYL, POLYCHLORO- see PJL750 BIPHENYL SELENIUM see PGH250 3,3, 4,4 -BIPHENYLTETRAMINE see BGK500 3,3, 4,4 -BIPHENYLTETRAMINE TETRAHYDROCHLORIDE see BGK750 N-(4-BIPHENYLYL)ACETAMIDE see PDY500 N,N -4,4 -BIPHENYLYLENEBISACETAMIDE see BFXOOO... 

DIAMINOBENZIDENE see BGK500 3,3 -DIAMINOBENZIDINE TETRAHYDROCHLORIDE see BGK750 6,6 -DIANnNO-m,m -BIPHENOL see DMI400 4,4 -DIAMINOBIPHENYL see BBXOOO p,p -DIAMINOBIPHENYL see BBXOOO 4,4 -DIAMINO-l,l -BIPHENYL see BBXOOO 4,4 -DIAMINOBIPHENYL-3,3 -DICARBOXYLIC ACID see BFX250... 

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