Collision-induced dissociation tandem mass spectrometry

CID-MS/MS flight mass spectrometry Collision-induced dissociation tandem mass... 

S.3.4.2. Collision-induced Dissociation/Translationally-driven Reactions Tandem-mass spectrometry can be used to determine the threshold energy for collision-induced dissociation (CID),... 

In most cases, ion activation in the reaction region or fragmentation zone is applied to increase the internal energy of the ions transmitted from the ion source. The most common means of ion activation in tandem mass spectrometry is collision-induced dissociation. CID uses gas-phase collisions between the ion and neutral target gas (such as helium, nitrogen or argon) to cause internal excitation of the ion and subsequent dissociation... 

Substituted tetrazoles reacting in the mass spectrometer with acyl ions afforded 2,5-disubstituted 1,3,4-oxadiazoles with nitrogen loss. Tandem mass spectrometry allowed for the collision-induced dissociation of the products. Chemical ionization was the better method to make the transformation. A scheme for the transformation of 5-substituted tetrazoles into 2,5-disubstituted 1,3,4-oxadiazoles was proposed (Scheme 1) <2001JMP1069>. The fragmentation patterns of monocyclic l,3,4-oxadiazolium-2-thiolates have been proposed by Ollis and Ramsden <1974J(P1)645>. 

Ma, Y.L. et al.. Characterization of fiavone and fiavonol aglycones by collision-induced dissociation tandem mass spectrometry. Rapid Commun. Mass Spectrom., 11, 1357, 1997. 

FAB and LSIMS are matrix-mediated desorption techniques that use energetic particle bombardment to simultaneously ionize samples like carotenoids and transfer them to the gas phase for mass spectrometric analysis. Molecular ions and/or protonated molecules are usually abundant and fragmentation is minimal. Tandem mass spectrometry with collision-induced dissociation (CID) may be used to produce abundant structurally significant fragment ions from molecular ion precursors (formed using FAB or any suitable ionization technique) for additional characterization and identification of chlorophylls and their derivatives. Continuous-flow FAB/LSIMS may be interfaced to an HPLC system for high-throughput flow-injection analysis or on-line LC/MS. 

S. B. Levery, M. S. Toledo, R. L. Doong, A. H. Straus, and H. K. Takahashi, Comparative analysis of ceramide structural modification found in fungal cerebrosides by electrospray tandem mass spectrometry with low energy collision-induced dissociation of Li + adduct ions, Rapid Commun. Mass Spectrom., 14 (2000) 551-563. 

In some cases the identification of proteins based on peptide masses is ambiguous or a suitable match cannot be found. Further information may be obtained by performing a collision-induced dissociation tandem mass spectrometry (CID MS/MS) experiment, in which the peptide molecular ions are fragmented in a collision cell and the resulting product ions are analyzed. These product ions often reveal sequence information that can then be used to identify the protein. 

C.S. Harden, A.P. Snyder and G.A. Eicemen, Determination of collision-induced dissociation mechanisms and cross-sections in organophos-phorus compounds by atmospheric pressure ionization tandem mass spectrometry, Org. Mass Spectrom., 28, 585-592 (1993). 

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