Tandem collision-induced dissociation

Tandem quadrupole and magnetic-sector mass spectrometers as well as FT-ICR and ion trap instruments have been employed in MS/MS experiments involving precursor/product/neutral relationships. Fragmentation can be the result of a metastable decomposition or collision-induced dissociation (CID). The purpose of this type of instrumentation is to identify, qualitatively or quantitatively, specific compounds contained in complex mixtures. This method provides high sensitivity and high specificity. The instrumentation commonly applied in GC/MS is discussed under the MS/MS Instrumentation heading, which appears earlier in this chapter. 

S.3.4.2. Collision-induced Dissociation/Translationally-driven Reactions Tandem-mass spectrometry can be used to determine the threshold energy for collision-induced dissociation (CID),... 

Medzihradszky, K.F., Campbell, J.M., Baldwin, M.A., Falick, A.M., Juhasz, P., Vestal, M.L., and Burlingame, A.L., The characteristics of peptide collision-induced dissociation using a high-performance MALDI-TOF/TOF tandem mass spectrometer, Anal. Chem., 72, 552, 2000. 

In most cases, ion activation in the reaction region or fragmentation zone is applied to increase the internal energy of the ions transmitted from the ion source. The most common means of ion activation in tandem mass spectrometry is collision-induced dissociation. CID uses gas-phase collisions between the ion and neutral target gas (such as helium, nitrogen or argon) to cause internal excitation of the ion and subsequent dissociation... 

Substituted tetrazoles reacting in the mass spectrometer with acyl ions afforded 2,5-disubstituted 1,3,4-oxadiazoles with nitrogen loss. Tandem mass spectrometry allowed for the collision-induced dissociation of the products. Chemical ionization was the better method to make the transformation. A scheme for the transformation of 5-substituted tetrazoles into 2,5-disubstituted 1,3,4-oxadiazoles was proposed (Scheme 1) <2001JMP1069>. The fragmentation patterns of monocyclic l,3,4-oxadiazolium-2-thiolates have been proposed by Ollis and Ramsden <1974J(P1)645>. 

K. F. Medzihradszky, J. M. Campbell, M. A. Baldwin, A. M. Falick, P. Juhasz, M. L. Vestal, and A. L. Burlingame. The Characteristics of Peptide Collision-Induced Dissociation Using a High-Performance MALDI-TOF/TOF Tandem Mass Spectrometer. Anal. Chem., 72(2000) 552-558. 

Papayannopoulos I.A. (1995), The interpretation of collision-induced dissociation tandem mass spectra of peptides, Mass Spectrom. Rev. 14, 49-73. 

Scans based on resonant ejection may either be carried out in a forward, i.e., from low to high mass, or a reverse manner. This allows for the selective storage of ions of a certain m/z value by elimination of ions below and above that m/z value from the trap. Thus, it can serve for precursor ion selection in tandem MS experminents. [156,158] Axial excitation can also be used to cause collision-induced dissociation (CID) of the ions as a result of numerous low-energy collisions with the helium buffer gas that is present in the trap in order to dampen the ion motion. [150,156] A substantial increase of the mass range is realized by reduction of both the RF frequency of the modulation voltage and the physical size of theQIT. [154,159,160]... 

Cornish, T.J. Cotter, R.J. Collision-Induced Dissociation in a Tandem Time-of-Flight Mass Spectrometer With Two... 

Yinon, J. Mass Spectral Fragm tation Pathways in Phtbalate Esters. A Tandem Mass Spectrometric Collision-Induced Dissociation Study. Org. Mass Spectrom. 1988,23,755-759. 

Ma, Y.L. et al.. Characterization of fiavone and fiavonol aglycones by collision-induced dissociation tandem mass spectrometry. Rapid Commun. Mass Spectrom., 11, 1357, 1997. 

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