Sytkowski

Wojchowski, D.M., and Sytkowski, A.J. (1986) Hybridoma production by simplified avidin-mediated electrofusion./. Immunol. Meth. 90, 173-177. 

Vitamin E is widespread in foods and is stored in the body so that deficiency states are very rare. A possible exception may be premature infants with very low fat stores. The concentration of a-tocopherol in cows milk ranges from 3.0 to 5.0 mg/L and is present at about the same level in human milk. While vitamin E has been shown to be essential for normal fertility in rats and other animals, it has never been proven to be necessary for human fertility. However, in recent years there has been renewed interest in the antioxidant function of vitamin E [e.g., in protecting the cardiovascular system (Sytkowski et al., 1990 Gurr, 1994)]. 

Sytkowski, P.A., Kannel, W.B., D Agostino, R.B. 1990. Changes in risk factors and the decline in mortality from cardiovascular disease the Framingham study. New Engl. J. Med. 322, 1635-1641. 

Mosterd A, D Agostino RB, Silbershatz H, Sytkowski PA, Kannel WB, Grobbee DE, Levy D. Trends in the prevalence of hypertension, antihypertensive therapy, and left ventricular hypertrophy from 1950 to 1989. N Engl J Med 1999 340(16) 1221-7. 

Sytkowski PA, D Agostino RB, Belanger A, Kannel WB. Sex and time trends in cardiovascular disease incidence and mortality The Framingham Heart Study, 1950-1989. Am J Epidemiol 1996 143 338-350. 

To this end, the spin labeling technique was used to probe the active site structure of LADH in various solvent systems. The spin label, SL-1, alkylated cysteine 46 (12), an amino acid in the active site of LADH that normally serves as a ligand to the catalytic zinc ion. LADH is a dimer, and each monomeric subunit contains one firmly-bound catalytic zinc ion. The position of enzyme-bound SL-1 was estimated by the spin label-spin probe technique (14.15). Cobalt (II) was employed as the spin probe, and the catalytic zinc in the enzyme s active site was replaced by Co + according to the procedure of Sytkowski and Vallee (16). The EPR spectrum of enzyme-bound SL-1 was then measured before and after replacement of the active-site zinc by cobalt from the decrease in spectral amplitude the average nitroxide-metal distance was determined to be 4.8 1.5 A (17). 

Lange LH, Sytkowski J, Vallee BL (1976) Human liver alcohol dehydrogenase purification, composition, and catalytic features. Biochemistry 15 4687-4693... 

Sytkowski, A.J. (2004) Erythropoietin Blood, Brain and Beyond. Wiley-VCH,Weinheim. 

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