Synthetic promoter libraries

Hammer K, Mijakovic I, Jensen PR (2006) Synthetic promoter libraries tuning of gene expression. Trends Biotechnol 24 53-55... 

Lezyk, M.J., Solem, C., and Jensen, P.R. (2014) Synthetic promoter libraries for Corynehacterium glutamicum. 

Keywords Metabohc engineering Standardization, orthogonality and modularity Synthetic and systems biology Synthetic gene networks Synthetic promoter libraries... 

Rud I, Jensen PR, Naterstad K, Axelsson LA (2006) Synthetic promoter library for constitutive gene expression in Lactobacillus plantarum. Microbiology 152 1011-1019 Rud I, Solem C, Jensen PR, Axelsson L, Naterstad K (2008) Co-factor engineering in lactobacflli effects of uncoupled ATPase activity on metabolic fluxes in Lactobacillus (L) plantarum and L. sakei. Metab Eng 10 207-215... 

Rytter JV, Helmark S, Solem C, Lezyk MJ, Jensen PR (manuscript in preparation) Synthetic promoter libraries for metabolic engineering of Corynebacterium glutamicum Siegele DA, Hu JC (1997) Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations. Proc Natl Acad Sci USA 94 8168-8172... 

Seghezzi, N., Amar, P., Koebmaim, B., Jensen, P.R. et al. (2011) The construction of a library of synthetic promoters revealed some specific features of strong Streptomyces promoters. Appl. Microbiol. Biotechnol, 90 (2), 615-623. 

Gertz et aL investigated a library of synthetic promoters to construct a thermodynamic model that could accurately predict gene expression levels from promoter sequence in yeast. A thermodynamic model was built under the assumptions that gene regulation is controlled completely by DNA-protein and protein-protein interactions. The model calculates the probability that RNA polymerase binds to the promoter and then assumes that this results in gene transcription and protein production (Gertz et al. 2009). 

Nitrilases (E.C. 3.5.5.1) promote the mild hydrolytic conversion of organonitriles directly to the corresponding carboxylic acids.19 However, less than 20 microbially derived nitrilases had been characterized at the start of this work, despite their potential synthetic value. The paucity of enzymes and the limited substrate scope of the handful of enzymes available have limited practical commercial development of nitrilase-catalyzed conversions, except in a few cases. Accordingly, we engaged in a discovery effort centered around exploiting the natural diversity available within our environmental DNA libraries and have discovered and characterized more than 200 new sequence unique nitrilases.20 All of the newly discovered nitrilases possess the conserved catalytic triad Glu-Lys-Cys that is characteristic for this enzyme class.19... 

Libraries of synthetic stationary-phase and stress promoters as a tool for fine-tuning of expression of recombinant proteins in Escherichia coli. J Biotechnol 120 25-37... 

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