Surface plasmon resonance model

C tyroki J., Homola J., Lambeck P.V., Musa S., Hoekstra H.J.W.M., Harris R.D., Wilinson J.S., Usievich B., Lyndin N.M., Theory and modelling of optical waveguide sensors utilising surface plasmon resonance, Sens. andActuat. B 1999 54 66-73. 

M. J. Hemaiz, J. M. de la Fuente, A. G. Barrientos, and S. Penades, A model system mimicking glycosphingolipid clusters to quantify carbohydrate selfinteractions by surface plasmon resonance, Angew. Chem. Int. Ed., 40 (2001) 2258-2261. 

Surface plasmon resonance studies were employed to measure the equilibrium constants and association and dissociation rate constants of bisnaphthalimide derivatives (20, 21) with hairpin DNA immobilized on the metal surface.123 The equilibrium constants were higher and the dynamics slower for compounds 20 and 21 when compared to the equilibrium constants and dynamics of the model monomer (19). The values for ka and kd were determined from the change in the surface plasmon resonance signal when, respectively, the ligand solution was flowed over the... 

The effect of structural modification to 22 on the binding efficiency and binding dynamics of hairpin DNA immobilized on metal surfaces were studied using surface plasmon resonance.124,125 The association and dissociation kinetics were analyzed using a sequential model for the binding of the imidazole containing polyamides... 

The optimal enhancement effect is observed when the localized surface plasmon resonance is tuned to the emission wavelength of a locally situated fluorophore [86]. This is consistent with the model suggesting a greatly increased efficiency for energy transfer from fluorophores to surface plasmons [78]. Since resonance energy transfer is involved, the important factors affecting the intensity of fluorescence emission must also be the orientation of the dye dipole moments relative to the... 

Certainly biomolecular NMR is not the single method which is important for hit identification in pharmaceutical research. It is always a combination of techniques and a team effort that leads to a successful drug. This can involve biologists (basic understanding, assay development, bio-informatics), chemists (both bench chemists and modelers), screening specialists (HTS/natural products) and spectroscopists (X-ray, optical methods, surface plasmon resonance, NMR). 

J Ctyroky, J. Homola, P. V. Lambeck, S. Musa, H.J.W.M. Hoekstra, R.D. Harris, J.S. Wdkinson, B. Usievich, N.M. Lyndin, Theory and modelling of optical waveguide sensors utihsing surface plasmon resonance, Sensors and Actuators B 54, 66-73 (1999). 

J. Ctyroky, J. Homola, M. Skalsky, Modelling of surface plasmon resonance waveguide sensor by complex mode expansion and propagation method, Opt. Quantum Electron. 29, 301-311 (1997). 

The mechanism of action of cyclotides is thought to involve membrane binding. This suggestion is supported by recent surface plasmon resonance studies showing that the cyclotides kalata B1 and B6 bind to lipids.122 The structure of kalata B1 in contact with DPC micelles, used as an NMR-friendly model... 

Combination of Surface Plasmon Resonance (SPR) and Optical Waveguide Spectroscopy (OWS) was used for the simultaneous determination of refractive index and film thickness of the hydrogel layers in the Kretschmann configuration [24], The resulting angle scans from the SPR instrument were fit to Fresnel calculations and different layers were represented using a simple box model. A detailed description of this process has been published previously [18]. 

Immunogenicity assays for investigating the frequency and consequences of antibody development against a protein therapeutic agent are typically based on an immunoassay technique (mostly ELIS As of various types). However, other assay formats are available such as radioimmunoprecipitation assay, surface plasmon resonance, and electrochemiluminescence [3]. Assays for measuring antibody response should be established in the early preclinical stage of development to estimate the value of the applied animal models (see Chapters 16 and 20). 

The instrument based upon surface plasmon resonance detection (5,9-111 is now routinely used to measure the binding of an antigen (or antibody) to an immobilized antibody (or antigen) in a flow cell. The technology relies on the covalent immobilization of one of the interacting species and the detection of the adsorbed analyte. The sophistication of this expensive instrumentation makes its use difficult for routine investigations in many laboratories. Furthermore, the models used to extract the rate constants are not always appropriate to the kinetic data analysis [ 12-141. 

Nitzan and Brus developed an analytical formula for the molecular absorption cross section given the model defined above [14]. Figure 9.2 is taken fi"om Ref. [13] and shows the calculated absorption cross section based on the model associated with the photodissociation of I2. (The I2 formed through the absorption process is very short lived.) Photodissociation predicted to be enhanced as the molecule is placed near a silver metal nanoparticle of radius a - 50 nm near the electronic transition resonance position of cat) 22,200 cm . If e eiai(co) is the dielectric fiinction for the metal, a small metal nanoparticle plasmon in air will have its dipolar surface plasmon resonance at frequency <24 such that [1]... 

Jensen, T.R., Schatz, G.C., and Van Duyne, R.P. (1999) Nanosphere lithography surface plasmon resonance spectrum of a periodic array of silver nanopartides by ultraviolet-visible extinction spectroscopy and electrodynamic modeling. Journal of Physical Chemistry B, 103, 2394—2401. 

Ctyroky J, Abdelmalek P, Ecke W, Usbeck K (1999a) Modelling of the surface plasmon resonance waveguide sensor with Bragg grating. Opt Quantum Electron 31 927-941... 

When the target protein of a small molecule is determined, it goes under validation with a number of biophysical, biochemical, and biological experiments, such as forward-affinity binding assay, reverse-affinity binding assay, small molecule-target protein docking model, surface plasmon resonance (SPR)... 

---