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Reverse-affinity binding assay

When the target protein of a small molecule is determined, it goes under validation with a number of biophysical, biochemical, and biological experiments, such as forward-affinity binding assay, reverse-affinity binding assay, small molecule-target protein docking model, surface plasmon resonance (SPR)... [Pg.86]

A large excess of affinity label is bound to both puri,fied tubulin and soluble brain extract as compared to the amount of [ H] colchicine retained." This could be explained by the existence of two classes of binding sites for colchicine. In one, the alkaloid is irreversibly bound and can also be detected by the ["H] colchicine binding assay. In the other, colchicine is reversibly bound and can be measured only using the covalent binding of ["Hj bromocolchicine. [Pg.571]

See other pages where Reverse-affinity binding assay is mentioned: [Pg.87]    [Pg.88]    [Pg.87]    [Pg.88]    [Pg.21]    [Pg.670]    [Pg.304]    [Pg.105]    [Pg.44]    [Pg.305]    [Pg.37]    [Pg.85]    [Pg.271]    [Pg.32]    [Pg.220]    [Pg.438]    [Pg.321]    [Pg.43]    [Pg.1576]    [Pg.47]    [Pg.112]    [Pg.195]    [Pg.394]    [Pg.182]    [Pg.283]    [Pg.284]    [Pg.254]    [Pg.311]    [Pg.192]    [Pg.728]    [Pg.52]    [Pg.1673]    [Pg.310]    [Pg.171]    [Pg.203]    [Pg.401]    [Pg.5]    [Pg.285]    [Pg.119]    [Pg.67]    [Pg.171]    [Pg.56]    [Pg.126]    [Pg.254]    [Pg.295]    [Pg.626]    [Pg.177]    [Pg.30]    [Pg.835]    [Pg.190]   
See also in sourсe #XX -- [ Pg.86 , Pg.87 ]



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Affinity assays

Binding affinity

Binding assays

Reverse assay

Reversible binding

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