Superspher Select

Zorbax SB Cl 8 Luna Nova-Pak Fluofix (EW Prodigy YMCC18 Hypersil BDS Prontosil Cl 8 Inertsil CDS 2 Prontosil AQ Repro-Sil CDS MP-Gel YMC AQ Purospher Nucleosil HD Discovery Cl 8 Repro-Sil AQ Supelcosil ABZ plus Jupiter Hypersil Elite Toso Haas TSK Inertsil QDS 3 Fluofix INW Ultrasep ES Discovery Cl 6 Nucleosil 50 Zorbax SB C8 Superspher LiChrosorb Superspher Select B Bondapak Nucleosil 100 LiChrosher Select B Supersorb QDS 2 LiChrospher Platinum Cl 8 Gromsil AB VYDAC Superisorb QDS 1 Platinum EPS Hypersil QDS Nucleosil Protect 1 Zorbax QDS... 

The similarity of the Cg phases LiChrospher Select B, Superspher Select B, and Zorbax SB Cg is clearly evident... 

As is known, liChrospher and Superspher are essentially identical, the only difference being in their partide size (5 im compared to 4 im). The hexagons are nearly identical. In the case of LiChrospher Select B and Superspher Select B, they are again nearly the same. 

It is advisable to always consider steric aspects. For this, one should test, e.g., a hydrophobic phase with a large pore diameter (Jupiter, Zorbax SB 300, Discovery Cjg, Hypersil BDS) as well as a phase with hydrophobic and/or polar properties and a small pore diameter, e.g., Nucleosil 50, Novapak, Superspher Select B. Remember. Even in the case of small molecules, the selectivity can be influenced by hindered/unhindered diffusion in different sized pores. 

Acesulfame-K, aspartame, saccharin Superspher RP-select B 4 /jin Nucleosil 100-5 Cl 8 20 mM phosphate buffer acetonitrile (9 1, v/v) UV 44... 

CF, TP, sweeteners, and additives HPLC-UV. Column Superspher RP-Select B. Mobile phase KH2P04-ACN (pH = 4.2-4.4 for sorbic and benzoic acids). 

Wad and Kramer [35] described an HPLC method for simultaneous determination of vigabatrin and gabapentin in serum and urine after precolumn derivatization with o-phthaldialdehyde and fluorimetric detection at 455 nm with excitation at 230 nm. A column (12.5 cm x 3.0 mm) of Superspher 60 RP-Selected B (5 /im) with acetonitrile in 20 mM 0.5 M KH7PO4 as a mobile phase (flow rate 0.7 ml/min). The day-to-day CV of vigabatrin in a pooled serum was 3.1%. The lower limit of detection was 0.5 /(mol/1 and the calibration graph was rectilinear from 1300 /tmol/1. 

Column LiChroCART column (125 X 2 mm i.d.) with Superspher 60 RP Select B equipped with LiChroCART 10-2 Superspher 60 RP Selected B guard column Detector APCI-LC-MS SIM monitoring ... 

Guard column 4x45 p.m LiChrospher 100 RP-18 Column 125 X 4 4 p,m Superspher 60 RP-select B (Merck)... 

Our opinion Also think about, for example, Hypersil ODS, LiChrospher Select B 60, Superspher or Cosmosil NAP additionally, for example, Jupiter, Zorbax SB 300 as well as Nucleosil 50 - then orthogonality is given for all cases. 

LiChrospher, Superspher, and Purospher e show similar selectivity patterns because of the similar high degree of coverage and the presence of polar groups on the surface. The doubtless existent metal ions and free silanol groups on the surface of LiChrospher and Superspher have no effect in this case. Also similar are Symmetry and Zorbax ODS, two phases that are markedly different in the case of the separation of ionic analytes. 

Group A Moderate surface area/hydrophobidty and shape selectivity, and low ion-exchange and hydrogen-bonding capacity, as exemplified by the new generation C jg silica phases, e.g., BetaBasic Cjg, ACE Cjg, Symmetry Cjg, XTerra MS Cjg, Purospher RPlSe, Superspher RPlSe, HyPURITY Cjg, Discovery Cjg (column nos. 7,1,101,116, 86, 99,42, and 16, respectively). 

Chemical purities were determined by HPLC on a Hewlett-Packard liquid chromatograph HP1090 series II using a LichroCART 125-4 Superspher 60-RP Select B column (Merck) and UV-detection at 254 nm wavelength. Separations were achieved with a linear gradient of Ig H3PO4 85% in 100 mL water and Ig H3PO4 85 % in 100 mL acetonitrile as mobile phase at a 1.2 mL/min. flow. 

---