Superspher

Fig. 7-9. Separation of amino acids after derivatization 5 with OPA and mercaptoethanol. Column Superspher 100 RP-18 (4 pm) LiChroCART 250-4, mobile phase 50 mM sodium acetate buffer pH 7.0/methanol, flowrate 1.0 ml min temperature 40 °C detection fluorescence, excitation 340 nm/emission 445 nm. Sample amino acid standard sample (Merck KGaA Application note W219180).

Fig. 3.159. IP-UV-DAD (a) and total-ion current (TIC)-ESI-MS (b) chromatogram of a 22-compound aromatic sulphonate standard mixture of 4 mf/1 in the SIM-NI mode. Injection volume 50 jA, fragmentation voltage 80 V. Superspher RP-18 fast and short column (75 x 4 mm i.d., 4 pm particle diameter). For peak identification see Table 3.44. Reprinted wih permission from R. Loos et al. [197].

Fig. 7. Reversed-phase HPLC chromatogram of an aqueous solution of iotrolan. a Low resolution [ODS-Hypersil, 5 pm, 250 x 4.6 mm water - methanol, 500 80 (v/v)]. b Low resolution (Superspher 100, 250x4 mm, water pH 3.4 (NH4-Formiate, 0.02 M), Na-heptane sulfonate (2 g L ), 1.5 mL min 40°C) Numbers 1-5 indicate the peak numbers, c High resolution (Superspher 100, 250x4 mm, water/methanol, 900 100, 0,4 mL min 40°C) Numbers 6-17 indicate the peak numbers...

LiChrospher /Superspher Si, CN, DIOL, NH2, Aluspher A1 Reversed-phase... 

Nonionic -----------------LiChrospher /Superspher RP-8, RP-18, Purospher RP-18e with water... 

LiChrospher /Superspher RP-18, RP-18e with buffer as eluent LiChrospher NH2, DIOL with buffer/organic eluent Purospher RP-18e... 

Fig. 9 ELSD chromatogram of the molecular species of bovine milk PC. Underivatized molecular species were separated on a 250 X 4.6-mm column packed with Superspher RP-100 at 75°C. The mobile phase contained 1-propanol, water, iso-octane, and ammonium acetate in a complex gradient. (Reprinted from Ref. 102 with the permission of Chromatographia.)...

Acesulfame-K, aspartame, saccharin Superspher RP-select B 4 /jin Nucleosil 100-5 Cl 8 20 mM phosphate buffer acetonitrile (9 1, v/v) UV 44... 

BA, SA Diet foods clarifying with Carrez SuperSpher RP-4 /zm LiChrosorb Phosphate buffer-acetonitrille (90 10) (v/v) 1 ml/min UV 220 nm 67... 

CF, TP, sweeteners, and additives HPLC-UV. Column Superspher RP-Select B. Mobile phase KH2P04-ACN (pH = 4.2-4.4 for sorbic and benzoic acids). 

Wad and Kramer [35] described an HPLC method for simultaneous determination of vigabatrin and gabapentin in serum and urine after precolumn derivatization with o-phthaldialdehyde and fluorimetric detection at 455 nm with excitation at 230 nm. A column (12.5 cm x 3.0 mm) of Superspher 60 RP-Selected B (5 /im) with acetonitrile in 20 mM 0.5 M KH7PO4 as a mobile phase (flow rate 0.7 ml/min). The day-to-day CV of vigabatrin in a pooled serum was 3.1%. The lower limit of detection was 0.5 /(mol/1 and the calibration graph was rectilinear from 1300 /tmol/1. 

Column LiChroCART column (125 X 2 mm i.d.) with Superspher 60 RP Select B equipped with LiChroCART 10-2 Superspher 60 RP Selected B guard column Detector APCI-LC-MS SIM monitoring ... 

Guard column 4x45 p.m LiChrospher 100 RP-18 Column 125 X 4 4 p,m Superspher 60 RP-select B (Merck)... 

Guard column 15 X 3 7 pm Brownlee Aquapore silica Colvunm 125 x 4 4 pm Superspher SI-60... 

HPLC Analysis. A Tracor liquid chromatograph equipped with a Model 951 pump unit, a 980 A solvent programmer, a 970 A detector and a computing integrator Model CI-lOB (LDC-Milton Roy) are used for analysis. Alkaloids are finally separated by reversed phase HPLC carried out on a LiChrosorb Superspher RP-18 column (Merck, particle size 4 pm, 125 mm X 4mm i.d.) at 25 °C by the mobile phase 2-propanol-acetonitrile-water (5 40 50) with 1% ammonium carbonate (flow rate Iml/min, UV detector at 280 nm). 

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