Superoxide dismutase gene, human

Ceballos-Picot, I., Nicole, A., Briand, P., Grimber, G., Delacourte, A., Defossez, A., Javoy-Agid, F., Lafon, M., Blouin, J.L. and Sinet, P.M. (1991). Neuronal-specific expression of human copper-zinc superoxide dismutase gene in transgenic mice animal model of gene dosage effects in Down s syndrome. Brain Res, 552, 198-214. 

Dl. Danciger, E., Dafni, N., Bernstein, Y., Laver-Rudich, Z., Neer, A., and Groner, Y., Human Cu,Zn superoxide dismutase gene family Molecular stmcture and characterization of four Cu,Zn superoxide dismutase-related pseudogenes. Proc. Natl. Acad. Sci. U.SA. 83,3619-3623... 

Mo Y, Barnett ME, Takemoto D et al (2007) Human serum albumin nanoparticles fw efficient delivery of Cu, Zn superoxide dismutase gene. Mol Vis 13 746-757... 

BEAS 2 B cells, a human bronchial epitheUal cell line, exposed to various concentrations of crocidolite showed an increase of DNA strand breaks as follows (% positive cells) after 2h exposure (2% at 10" g/cm and 3 % at 5 x 10" g/cm ), after 24 h exposure (16% at 10" g/cm and 20% at 5xl0" g/ cm ) (Gillissen et al. 1996). In contrast, even at highest concentrations (24 h exposure) man-made mineral fibre basalt wool did not cause any increase of DNA strand breaks. Scanning electron microscopy confirmed that both fibre types were in part incorporated into the cells. Manganese superoxide dismutase gene expression was induced by 2ji UICC crocidolite/cm (Jaworska et al. 1997). 

Sinet, P.-M. SOD genes in humans Chromosome localization and electrophoretic variants. In Superoxide and Superoxide Dismutases (Michelson, A. M., McCord, J. M., Fridovich, I., eds.), London-New York-San Francisco, Academic Press, 1977, pp. 459-465... 

This is a widely studied cell line derived from a human cervix adenocarcinoma (Gey et al, 1952). The cells are epithelial-like in morphology and are susceptible to polio virus type 1 and adenovirus type 3. HeLa cells are used for the expression of recombinant proteins, including mouse metallothionein 1 gene, human Cu/Zn superoxide dismutase and hepatitis B surface antigen. They have been widely used as an in vitro model system because of the ease with which they can be cultivated but one drawback of this is that the cell line has been responsible for widespread contamination of other cell lines (Nelson-Rees et al, 1981). 

Low-molecular-weight catalysts which mimic a natural enzymic function syn-Zymes) have potential utility for the treatment of diseases characterized by the overproduction of a potentially deleterious metabolic by-product or foreign gene product. The discovery and development of pentaaza macrocyclic ligand complexes of manganese(II) as functional mimics of superoxide dismutase (SOD) enzymes and their potential utility as human pharmaceutical agents is described. 

El. Elroy-Stein, O., and Groner, Y., Impaired neurotransmitter uptake in PC12 cells overexpressing human Cu/Zn-superoxide dismutase—Implication for gene dosage effects in Down syndrome. Cell (Cambridge, Mass.) 52, 259-267 (1988). 

Human mutations in the gene encoding the copper/zinc form of superoxide dismutase have been shown to cause amyotrophic lateral sclerosis, a neurodegenerative disorder better known as Lou Gehrig s disease. 

Turunen, P., Puhakka, H.L., Heikura, T, Romppanen, E., Inkala, M Leppanen, 0., and Yla-Herttuala, S. (2006) Extracellular superoxide dismutase with vaccinia virus anti-inflammatory protein 35K or tissue inhibitor of metaDoproteinase-l Combination gene therapy in the treatment of vein graft stenosis in rabbits. Human Gene Therapy, 17, 405—414. 

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