5-Sulfosalicylate

Precipitation of Fe(IIl) compounds from acid solutions as the pH increases above 2.2 is a particular problem. Complexing agents that have been used include 5-sulfosalicylic acid and citric acid (136) dihydroxymaleic acid (137) ethylenediaminetetraacetic acid (138) lactic acid (138) blends of hydroxylamine hydrochloride, citric acid, and glucono-delta-lactone (139) nitriloacetic acid blends of citric acid and acetic acid lactic acid and gluconic acid (140). 

The electrophoresis is finished when the tracking dye has reached about 5 mm above the bottom of the gel. Turn off the power supply, open the cassette carefully, and submerse the gel into a tenfold of gel volume of fixation solution [ 15% trichloroacetic acid (w/v), 15% 5-sulfosalicylic acid (w/v), or glacial acetic acid/methanol/H2 O (1 3 6, v/v/v)] or mount the gel into the electrotransfer apparatus. 

Fix the gel thoroughly either in TCA or 5-sulfosalicylic acid (cf. Protocol 2.1.1) after electrophoresis to remove urea prior to staining. 

Gels may he fixed in Soln. B, i.e., a previous fixation by TCA or 5-sulfosalicylic acid is mostly not necessary. 

Reaction with cold nitric acid results primarily in the formation of 5-nitrosalicylic acid [96-97-9]. However, reaction with fuming nitric acid results in decarboxylation as well as the formation of 2,4,6-trinitrophenol [88-89-1] (picric acid). Sulfonation with chlorosulfonic acid at 160°C yields 5-sulfosalicylic acid [56507-30-3]. At higher temperatures (180°C) and with an excess of chlorosulfonic acid, 3,5-disulfosalicylic acid forms. Sulfonation with liquid sulfur trioxide in tetrachloroethylene leads to a nearly quantitative yield of 5-sulfosalicylc acid (1). 

Sulfosalicylic acid is prepared by heating 10 parts of salicylic acid with 50 parts of concentrated sulfuric acid, by chlorosulfonation of salicylic acid and subsequent hydrolysis of the acid chloride, or by sulfonation with liquid sulfur trioxide in tetrachloroethylene. It is used as an intermediate in the production of dyestuffs, grease additives, catalysts, and surfactants. It is also useful as a colorimetric reagent for ferric iron and as a reagent for albumin. Table 9 shows the physical properties of salicylic acid derivatives. 

The glycolate Cr(C2H303)2-H20,213 salicylate Cr(C6H4(0)C02)-3H20 and tartrate Cr(C02CH0HCH0HC02)3 have been prepared. Stability constants have been determined for complexation of Cr11 with salicylate and 5-sulfosalicylate (Table 39). 

SALICYLIC ACID AND RELATED COMPOUNDS] (Vol 21) 5-Sulfosalicylic acid [97-05-2]... 

The esterification of steroids is a step in the synthesis of modified hormones. H2PW 2O40 and 25% HjPW CWSiOz (1-9%) in CH3CN (at 313-353 K) give a quantitative yield (363). The activities of the heteropolyacids are close to that of HC104 and much greater than that of 5-sulfosalicylic acid. 

Mixed complex formation kinetics between LnEDTA and L, where L stands for 5-sulfosalicylate [74], 8-hydroxy quinoline-5 sulfonate and picolinate [75] were studied and two pathways namely acid-dependent and acid-independent pathways have been identified. The overall reaction stoichiometry may be written as... 

To analyze free amino acids in plasma or tissue homogenates, it is necessary to remove proteins and peptides present in solution. The most widely used deproteinization method is precipitation with 5-sulfosalicylic acid followed by centrifugation for separating the precipitate. In comparison to other precipitation agents such as trichloroacetic acid, perchloric acid, picrinic acid, or acetonitrile, the best results with respect to completeness of precipitation are obtained with 5-sulfosalicylic acid [39]. Other deproteinization methods comprise ultrafiltration and ultracentrifugation [40], which have only recently been considered as sample preparation methods for amino acid analysis. 

Free amino acids were analyzed by first dissolving san5>les (0.05-0.1 g/mL) in 0.2 N citrate buffer, pH 2.2, and the mixture shaken for 15 min at room tenq)erature. Mixture was centrifuged at 7000 x g for 5 minutes and supernatant deproteinized with 3.5 % sulfosalicylic acid solution by mixing at a 1 1 ratio. Solution was chilled and shaken to maximize precipitation of imwanted protein. After thawing, the solution was centrifuged at 7000 x g for 5 minutes. 

Jiang, L., Gao, L., and Liu, Y, Adsorption of salicylic acid, 5-sulfosalicylic acid and Tiron at the alumina-water interface. Colloids Surf. A, 211, 165. 2002. 

The successful application of 5-sulfosalicylic acid in ion exchange separations was demonstrated by Fritz and Palmer [15]. At an appropriate pH, they found that alumi-num(III), iron(III), uranium(VI), and vanadium(IV) are complexed and pass quickly through a cation exchange column. A number of other cations, including the rare earths, are not complexed and are retained on the column. Their application was simply a group separation, and no chromatographic separation of individual metal ions was involved. 

Salicylic acid 5-Sulfosalicylic acid 2,4-Pentanedione Ferroin Gupta Srinivasulu (1982) / Treindle Fabian (1980) 1 Treindle Kaplan (1981)... 

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