Sugar-lectin interaction

Another interesting and recent application of ACE is the fluorescence-enhanced competition assay for the detection of sugar-lectin interactions (77). Because glucose competes with the fluorescently labeled dextran for the lectin-binding sites, the relative fluorescent intensity due to the displaced dextran is proportional to the concentration of glucose. 

M Hong, A Cassely, Y Mechref, MV Novothy. Sugar-lectin interactions investigated through affinity capillary electrophoresis. J Chromatogr B 752 207-216, 2001. 

Monsigny M, Mayer R, Roche AC (2000) Sugar-lectin interactions sugar clusters, lectin multivalency and avidity. Carbohydr Lett 4 35-52... 

Lipid Domains, Chemistry of Neoglycoproteins, Chemistry of Sugar-Lectin Interactions in Cell Adhesion Glycomimetics... 

Monsigny, M., Roche, A.C., Sene, C., Maget-Dana, R. and Delmotte, F, 1980, Sugar-lectin interactions How does wheat-germ agglutinin bind sialoglycoconjugates European Journal of Biochemistry 104, 147-153. 

M. Monsigny, A. C. Roche, C. Sene, R. Maget Dana, and F. Delmotte, Sugar-lectin interactions—how does wheat-germ agglutinin bind sialoglycoconjugates , Eur. J. Biochem., 104 (1980) 147-153. 

Anzai, J. Kobayashi, Y. Constraction of multilayer thin films of enzymes by means of sugar-lectin interactions. Langmuir 2000, 16, 2851. 

R 459 D. Neumann, C.-M. Lehr, H.-P. Lenhof and O. Kohlbacher, Computational Modeling of the Sugar-Lectin Interaction , Adv.Drug Deliver.Rev., 2004,56,437... 

Many lectins combine preferentially with a single sugar structure, for example g-galactose or L-fucose. For some lectins the specificity is broader and includes several closely related sugars, e.g., D-man-nose, g-glucose and g-arabinose other lectins-interact only with complex carbohydrate structures such as those that occur in glycoproteins, glycolipids,... 

Lectin name Source Sugar specificity Interacting antibody... 

Systems that have been studied with this method include the binding of lectins with sugars, the interactions of drugs and amino acids with serum albumin, and the kinetics of protein-based chiral stationary phases. 

Sugar-lectin specific interaction is an important cellular phenomenon. Li et al. [64] linked L-fucose to chitosan, which can act as a somatic agglutinin to induce bacteria aggregation. The enhanced antimicrobial activity of chitosan-L-fucose is attributed to the specific recognition and binding of the L-fucose moiety with PA-Il lectin on Pseudomonas aeruginosa surface. 

Theoretical aspects of affinity electrophoresis have been discussed and the applicability of the technique for determination of dissociation constants of protein-ligand complexes demonstrated. The pH dependence of lectin interactions with sugars has been determined by affinity electrophoresis. Association constants of dextrans and isomaltose oligosaccharides for myeloma anti-dextran have been determined using the technique. 

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