Staphylococcus aureus attachment

Figure 11.4a,b show the microcapsules as initially formed with Listeria monocytogenes and Staphylococcus aureus attached to the forming polyamide capsule wall. The bacteria, respectively rod and coccoid shaped, were clearly visible on the outside surface of the capsules when labelled with ethidium bromide, as evidenced by confocal optical sections. Variation of the initial bacterial concentration and... 

In reconstitution experiments, the self-assembly of the pore-forming protein a-hemolysin of Staphylococcus aureus (aHL) [181-183] was examined in plain and S-layer-supported lipid bilayers. Staphylococcal aHL formed lytic pores when added to the lipid-exposed side of the DPhPC bilayer with or without an attached S-layer from B coagulans E38/vl. The assembly of aHL pores was slower at S-layer-supported compared to unsupported folded membranes. No assembly could be detected upon adding aHL monomers to the S-layer face of the composite membrane. Therefore, the intrinsic molecular sieving properties of the S-layer lattice did not allow passage of aHL monomers through the S-layer pores to the lipid bilayer [142]. 

UDPG was the first of a whole new class of nucleotides, in which the uridine diphosphate group is attached to a monosaccharide. Two important members of the class are uridine diphosphate glucuronic acid, the intermediate in the synthesis of glucuronides and of many other compounds, and uridine diphosphate N-acetylglucosamine which is an intermediate in the synthesis of mucopolysaccharides. Uridine diphosphate muramic acid was isolated by Park (P2) from Staphylococcus aureus... 

Fibronectin is an extracellular matrix protein that mediates a variety of cellular effects. It is important in cell-cell and cell-substratum interactions ( 3.9), mediates reticuloendothelial cell activity and binds both to Clq (the first component of complement) and to bacteria. It also increases the tu-mouricidal activity of macrophages and activates complement receptors, by regulating the binding of C3b-coated particles to neutrophils. It may mediate attachment of Staphylococcus aureus to neutrophils and may also play a role as an adhesion factor, promoting the adhesion of neutrophils to surfaces. Fibronectin mRNA (8.7-8.8 kb) is detected only at low levels in... 

For these experiments, they used a more well-defined method for attaching the myosin to the beads. The beads were clumps of killed bacterial (Staphylococcus aureus) cells. These cells have a protein on their surface that binds to the Fc region of antibody molecules (Fig. 5-2la). The antibodies, in turn, bind to several (unknown) places along the tail of the myosin molecule. When bead-antibody-myosin complexes were prepared with intact myosin molecules, they would move along Nitella actin fibers in the presence of ATP. 

If the antibody is unable to cross-link or aggregate the antigen of interest to the extent needed to induce precipitation of the complex (as is often the case with monoclonal antibodies), an alternative method may be employed. Protein A from Staphylococcus aureus is known to have a high affinity for immunoglobulins. If a soluble antigen-antibody complex is incubated with an insoluble matrix covalently conjugated with Protein A, the complex will be captured by the Protein A covalently attached to the matrix (Fig. IV-9). Because Protein A... 

The synthesis and biocidal efficacies of two PEG-N-halamine polymers were studied. The N-halamines were classified as dichlorohydantoins and chloroimidazolidin-4-ones, which were attached to a methoxy-PEG terminated amine. A1000 ppm solution of each N-halamine polymer was prepared in water and it was demonstrated that Staphylococcus aureus was inactivated when it was in contact with these polymeric solutions for ten minutes. The stability of these solutions was determined over prolonged periods of time, as well as the biocidal efficacies. These polymeric materials showed commercial potential as surface active biocides if the PEG moiety could be anchored to a srrrface. 22 refs. 

Biotinylated bacterial antibodies linked via an avidin-biotin-hexanediamine spacer arm to carbon felt disks and attached to a platinum electrode Staphylococcus aureus and Escherichia coli [56]... 

To increase the purity or to concentrate an antibody solution, it may be purified. Purification is done with a range of techniques applied to whole serum, supernatant, or ascites fluid. At the first level, the purified Ig will be separated from other serum proteins and will select all IgGs including the IgG of interest and other IgG molecules. These purification steps can be done by using ammonium sulfate to precipitate the Ig molecules or it can be done by binding antibodies to a Protein A and/or Protein G columns. Proteins A and G are produced by the bacteria. Staphylococcus aureus, and bind to different species and subclasses of antibodies by the Fc receptor. After the antibodies have attached, they are washed out by changing the buffer. 

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